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Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme.

Publication ,  Journal Article
Perrotta, AT; Been, MD
Published in: Nucleic Acids Res
August 25, 1993

Three models for the secondary structure of the hepatitis delta virus (HDV) antigenomic self-cleaving RNA element were tested by site-directed mutagenesis. Two models in which bases 5' to the cleavage site are paired with sequence at the 3' end of the element were both inconsistent with the data from the mutagenesis. Specifically, mutations in the 3' sequence which decrease self-cleavage activity could not be compensated by base changes in the 5' sequence as predicted by these models. The evidence was consistent with a third model in which the 3' end pairs with a portion of a loop within the ribozyme sequence to generate a pseudoknot structure. This same pairing was also required to generate higher rates of cleavage in trans with a 15-mer ribozyme, thus ruling out a proposed hammerhead-like 'axehead' model for the HDV ribozyme.

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Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

August 25, 1993

Volume

21

Issue

17

Start / End Page

3959 / 3965

Location

England

Related Subject Headings

  • RNA, Viral
  • RNA, Catalytic
  • Nucleic Acid Conformation
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Kinetics
  • Hepatitis Delta Virus
  • Developmental Biology
  • DNA, Viral
  • Base Sequence
 

Citation

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Perrotta, A. T., & Been, M. D. (1993). Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme. Nucleic Acids Res, 21(17), 3959–3965. https://doi.org/10.1093/nar/21.17.3959
Perrotta, A. T., and M. D. Been. “Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme.Nucleic Acids Res 21, no. 17 (August 25, 1993): 3959–65. https://doi.org/10.1093/nar/21.17.3959.
Perrotta AT, Been MD. Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme. Nucleic Acids Res. 1993 Aug 25;21(17):3959–65.
Perrotta, A. T., and M. D. Been. “Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme.Nucleic Acids Res, vol. 21, no. 17, Aug. 1993, pp. 3959–65. Pubmed, doi:10.1093/nar/21.17.3959.
Perrotta AT, Been MD. Assessment of disparate structural features in three models of the hepatitis delta virus ribozyme. Nucleic Acids Res. 1993 Aug 25;21(17):3959–3965.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

August 25, 1993

Volume

21

Issue

17

Start / End Page

3959 / 3965

Location

England

Related Subject Headings

  • RNA, Viral
  • RNA, Catalytic
  • Nucleic Acid Conformation
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Kinetics
  • Hepatitis Delta Virus
  • Developmental Biology
  • DNA, Viral
  • Base Sequence