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Evidence that genomic and antigenomic RNA self-cleaving elements from hepatitis delta virus have similar secondary structures.

Publication ,  Journal Article
Rosenstein, SP; Been, MD
Published in: Nucleic Acids Res
October 11, 1991

The two sequences that define the self-cleaving elements from the genomic and antigenomic RNA of hepatitis delta virus were folded into secondary structures with similar features. Evidence in support of the two models was obtained from limited ribonuclease digestion of genomic and antigenomic RNA fragments containing the sequence 3' of the cleavage site. Under conditions where the rates of self-cleavage are enhanced by addition of 5 M urea (2-10 mM Mg2+ at 37 degrees C), ribonucleases T1, U2, A and V1 generated digestion patterns consistent with the proposed RNA structures. The evidence for a relatively stable structure in urea when Mg2+ is present suggests that denaturant-enhanced rates of self-cleavage could result from destabilization of competing inactive structures.

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Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

October 11, 1991

Volume

19

Issue

19

Start / End Page

5409 / 5416

Location

England

Related Subject Headings

  • Urea
  • Ribonuclease, Pancreatic
  • Ribonuclease T1
  • RNA, Viral
  • RNA, Catalytic
  • Nucleic Acid Conformation
  • Models, Molecular
  • Magnesium
  • Kinetics
  • Hepatitis Delta Virus
 

Citation

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Rosenstein, S. P., & Been, M. D. (1991). Evidence that genomic and antigenomic RNA self-cleaving elements from hepatitis delta virus have similar secondary structures. Nucleic Acids Res, 19(19), 5409–5416. https://doi.org/10.1093/nar/19.19.5409
Rosenstein, S. P., and M. D. Been. “Evidence that genomic and antigenomic RNA self-cleaving elements from hepatitis delta virus have similar secondary structures.Nucleic Acids Res 19, no. 19 (October 11, 1991): 5409–16. https://doi.org/10.1093/nar/19.19.5409.
Rosenstein, S. P., and M. D. Been. “Evidence that genomic and antigenomic RNA self-cleaving elements from hepatitis delta virus have similar secondary structures.Nucleic Acids Res, vol. 19, no. 19, Oct. 1991, pp. 5409–16. Pubmed, doi:10.1093/nar/19.19.5409.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

October 11, 1991

Volume

19

Issue

19

Start / End Page

5409 / 5416

Location

England

Related Subject Headings

  • Urea
  • Ribonuclease, Pancreatic
  • Ribonuclease T1
  • RNA, Viral
  • RNA, Catalytic
  • Nucleic Acid Conformation
  • Models, Molecular
  • Magnesium
  • Kinetics
  • Hepatitis Delta Virus