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Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.

Publication ,  Journal Article
Been, MD; Champoux, JJ
Published in: Nucleic Acids Res
December 20, 1980

The DNA nicking-closing enzyme (type I topoisomerase) from rat liver nuclei breaks single-stranded DNA. The broken strand contains a 5'-hydroxyl and tightly bound protein. The stability of this protein-DNA complex to high salt, alkali and detergent suggests a covalent linkage between the DNA and the enzyme. The observed breakage of single-stranded DNA occurs at neutral pH prior to treatment with alkali or detergent, indicating that the breakage may be the result of an interrupted nicking and closing cycle. The resulting covalent complex could represent a reaction intermediate in the overall nicking-closing reaction.

Duke Scholars

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

December 20, 1980

Volume

8

Issue

24

Start / End Page

6129 / 6142

Location

England

Related Subject Headings

  • Ultracentrifugation
  • Rats
  • Protein Binding
  • Liver
  • Kinetics
  • Developmental Biology
  • DNA, Viral
  • DNA, Single-Stranded
  • DNA Topoisomerases, Type I
  • Bacteriophage phi X 174
 

Citation

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MLA
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Been, M. D., & Champoux, J. J. (1980). Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex. Nucleic Acids Res, 8(24), 6129–6142. https://doi.org/10.1093/nar/8.24.6129
Been, M. D., and J. J. Champoux. “Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.Nucleic Acids Res 8, no. 24 (December 20, 1980): 6129–42. https://doi.org/10.1093/nar/8.24.6129.
Been, M. D., and J. J. Champoux. “Breakage of single-stranded DNA by rat liver nicking-closing enzyme with the formation of a DNA-enzyme complex.Nucleic Acids Res, vol. 8, no. 24, Dec. 1980, pp. 6129–42. Pubmed, doi:10.1093/nar/8.24.6129.

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

December 20, 1980

Volume

8

Issue

24

Start / End Page

6129 / 6142

Location

England

Related Subject Headings

  • Ultracentrifugation
  • Rats
  • Protein Binding
  • Liver
  • Kinetics
  • Developmental Biology
  • DNA, Viral
  • DNA, Single-Stranded
  • DNA Topoisomerases, Type I
  • Bacteriophage phi X 174