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Metabolism of 22-oxacalcitriol by a vitamin D-inducible pathway in cultured parathyroid cells.

Publication ,  Journal Article
Brown, AJ; Berkoben, M; Ritter, C; Kubodera, N; Nishii, Y; Slatopolsky, E
Published in: Biochem Biophys Res Commun
December 15, 1992

Catabolism of 22-oxacalcitriol (OCT) in parathyroid cells was compared to that of the parent hormone, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. Catabolism of both compounds was greatly accelerated by pretreatment of the cells with 1,25-(OH)2D3 or OCT. The rate of degradation of OCT was slightly greater than that of 1,25-(OH)2D3. Excess unlabeled OCT or 1,25-(OH)2D3 inhibited metabolism of both tritiated substrates. Ketoconazole, a cytochrome P450 inhibitor, blocked catabolism of both compounds. The major OCT metabolite appeared to be 1,20-dihydroxy-22,23,24,25,26,27-hexanor-vitamin D3 which was not active in suppressing PTH secretion. We conclude that OCT appears to be metabolized by the same vitamin D-inducible side chain oxidation pathway that catabolizes other vitamin D compounds and that its higher than expected suppression of PTH secretion is not due to slower cellular metabolism.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

December 15, 1992

Volume

189

Issue

2

Start / End Page

759 / 764

Location

United States

Related Subject Headings

  • Parathyroid Glands
  • Kinetics
  • Chromatography, High Pressure Liquid
  • Cells, Cultured
  • Cattle
  • Calcitriol
  • Biochemistry & Molecular Biology
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
 

Citation

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Brown, A. J., Berkoben, M., Ritter, C., Kubodera, N., Nishii, Y., & Slatopolsky, E. (1992). Metabolism of 22-oxacalcitriol by a vitamin D-inducible pathway in cultured parathyroid cells. Biochem Biophys Res Commun, 189(2), 759–764. https://doi.org/10.1016/0006-291x(92)92266-z
Brown, A. J., M. Berkoben, C. Ritter, N. Kubodera, Y. Nishii, and E. Slatopolsky. “Metabolism of 22-oxacalcitriol by a vitamin D-inducible pathway in cultured parathyroid cells.Biochem Biophys Res Commun 189, no. 2 (December 15, 1992): 759–64. https://doi.org/10.1016/0006-291x(92)92266-z.
Brown AJ, Berkoben M, Ritter C, Kubodera N, Nishii Y, Slatopolsky E. Metabolism of 22-oxacalcitriol by a vitamin D-inducible pathway in cultured parathyroid cells. Biochem Biophys Res Commun. 1992 Dec 15;189(2):759–64.
Brown, A. J., et al. “Metabolism of 22-oxacalcitriol by a vitamin D-inducible pathway in cultured parathyroid cells.Biochem Biophys Res Commun, vol. 189, no. 2, Dec. 1992, pp. 759–64. Pubmed, doi:10.1016/0006-291x(92)92266-z.
Brown AJ, Berkoben M, Ritter C, Kubodera N, Nishii Y, Slatopolsky E. Metabolism of 22-oxacalcitriol by a vitamin D-inducible pathway in cultured parathyroid cells. Biochem Biophys Res Commun. 1992 Dec 15;189(2):759–764.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

December 15, 1992

Volume

189

Issue

2

Start / End Page

759 / 764

Location

United States

Related Subject Headings

  • Parathyroid Glands
  • Kinetics
  • Chromatography, High Pressure Liquid
  • Cells, Cultured
  • Cattle
  • Calcitriol
  • Biochemistry & Molecular Biology
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology