Skip to main content
Journal cover image

Domain-specific effector interactions within the central cavity of human adult hemoglobin in solution and in porous sol-gel matrices: evidence for long-range communication pathways.

Publication ,  Journal Article
Peterson, ES; Shinder, R; Khan, I; Juczszak, L; Wang, J; Manjula, B; Acharya, SA; Bonaventura, C; Friedman, JM
Published in: Biochemistry
April 2004

The water-filled central cavity of human adult hemoglobin (Hb A) is the binding or interaction site for many different allosteric effectors. Oxygen binding titrations reveal that pyrenetetrasulfonate (PyTS), a fluorescent analogue of 2,3-diphosphoglycerate, behaves like an allosteric effector. The ligation state, pH, and concentrations of other effectors (IHP, L35, and chloride) alter PyTS fluorescence for both solution-phase and sol-gel-encapsulated Hb samples. These conditions also alter the resonance Raman spectra and rates of geminate recombination of CO-ligated Hb. Together, these results demonstrate that there are conformational and functional consequences resulting from interactions between specific domains of the central cavity and individual effectors as well as from long-range synergistic effects that are mediated through the central cavity.

Duke Scholars

Published In

Biochemistry

DOI

EISSN

1520-4995

ISSN

0006-2960

Publication Date

April 2004

Volume

43

Issue

16

Start / End Page

4832 / 4843

Related Subject Headings

  • Spectrum Analysis, Raman
  • Spectrometry, Fluorescence
  • Solutions
  • Pyrenes
  • Protein Structure, Tertiary
  • Protein Structure, Quaternary
  • Protein Conformation
  • Phenylurea Compounds
  • Oxygen
  • Ligands
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Peterson, E. S., Shinder, R., Khan, I., Juczszak, L., Wang, J., Manjula, B., … Friedman, J. M. (2004). Domain-specific effector interactions within the central cavity of human adult hemoglobin in solution and in porous sol-gel matrices: evidence for long-range communication pathways. Biochemistry, 43(16), 4832–4843. https://doi.org/10.1021/bi035481o
Peterson, Eric S., Roman Shinder, Imran Khan, Laura Juczszak, Jiaqian Wang, Belur Manjula, Seetharama A. Acharya, Celia Bonaventura, and Joel M. Friedman. “Domain-specific effector interactions within the central cavity of human adult hemoglobin in solution and in porous sol-gel matrices: evidence for long-range communication pathways.Biochemistry 43, no. 16 (April 2004): 4832–43. https://doi.org/10.1021/bi035481o.
Peterson ES, Shinder R, Khan I, Juczszak L, Wang J, Manjula B, Acharya SA, Bonaventura C, Friedman JM. Domain-specific effector interactions within the central cavity of human adult hemoglobin in solution and in porous sol-gel matrices: evidence for long-range communication pathways. Biochemistry. 2004 Apr;43(16):4832–4843.
Journal cover image

Published In

Biochemistry

DOI

EISSN

1520-4995

ISSN

0006-2960

Publication Date

April 2004

Volume

43

Issue

16

Start / End Page

4832 / 4843

Related Subject Headings

  • Spectrum Analysis, Raman
  • Spectrometry, Fluorescence
  • Solutions
  • Pyrenes
  • Protein Structure, Tertiary
  • Protein Structure, Quaternary
  • Protein Conformation
  • Phenylurea Compounds
  • Oxygen
  • Ligands