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The phenotype of human placental macrophages and its variation with gestational age.

Publication ,  Journal Article
Goldstein, J; Braverman, M; Salafia, C; Buckley, P
Published in: Am J Pathol
December 1988

The antigenic phenotype of human villous stromal macrophages (M phi s) from first and third trimester placentas was analyzed using a large number of monoclonal antibodies (MAbs) to monocyte (Mo)/M phi-associated cell membrane determinants. The purpose of this study was to investigate M phi phenotypic heterogeneity to create a database for the correlation of M phi phenotype with specific immunologic functions. The results showed that villous stromal mononuclear cells express many cell surface antigens found on Mo and M phi s and that they are morphologically diverse, ranging in appearance from classic Hofbauer cells to spindle-shaped cells with long cytoplasmic processes. Villous stromal M phi s were the numerically dominant cell type in this structure and exhibited some major phenotypic differences from M phi s in other tissues. Comparison of first- and third-trimester placentas revealed variation in antigen expression with increasing gestational age, in particular of class II major histocompatibility complex (MHC) determinants: HLA-DR and HLA-DP antigen density was low on first-trimester villous M phi s and much higher on third-trimester M phi s while HLA-DQ was undetectable in the first trimester but present on cells in third trimester placentas. The CD1 (T6) antigen, found on Langerhans (LH) cells and cortical thymocytes, was detected on villous M phi s by two thirds of the MAbs directed against different epitopes on this determinant. Furthermore, comparison with similar studies of lymphoid tissues showed that villous M phi s and dendritic cells share the expression of a number of other cell surface antigens. Finally, it was shown that M phi s in first- and third-trimester villi exhibit strong reactivity with MAbs (Leu 3a,b) to the CD4 antigen that serves as the receptor for the human immunodeficiency virus (HIV), suggesting that these cells may be a portal of entry or reservoir for this virus in the fetuses of pregnant, HIV+ women.

Duke Scholars

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

December 1988

Volume

133

Issue

3

Start / End Page

648 / 659

Location

United States

Related Subject Headings

  • Receptors, Virus
  • Receptors, HIV
  • Receptors, Fc
  • Receptors, Complement
  • Pregnancy
  • Placenta
  • Phenotype
  • Pathology
  • Macrophages
  • Immunohistochemistry
 

Citation

APA
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ICMJE
MLA
NLM
Goldstein, J., Braverman, M., Salafia, C., & Buckley, P. (1988). The phenotype of human placental macrophages and its variation with gestational age. Am J Pathol, 133(3), 648–659.
Goldstein, J., M. Braverman, C. Salafia, and P. Buckley. “The phenotype of human placental macrophages and its variation with gestational age.Am J Pathol 133, no. 3 (December 1988): 648–59.
Goldstein J, Braverman M, Salafia C, Buckley P. The phenotype of human placental macrophages and its variation with gestational age. Am J Pathol. 1988 Dec;133(3):648–59.
Goldstein, J., et al. “The phenotype of human placental macrophages and its variation with gestational age.Am J Pathol, vol. 133, no. 3, Dec. 1988, pp. 648–59.
Goldstein J, Braverman M, Salafia C, Buckley P. The phenotype of human placental macrophages and its variation with gestational age. Am J Pathol. 1988 Dec;133(3):648–659.
Journal cover image

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

December 1988

Volume

133

Issue

3

Start / End Page

648 / 659

Location

United States

Related Subject Headings

  • Receptors, Virus
  • Receptors, HIV
  • Receptors, Fc
  • Receptors, Complement
  • Pregnancy
  • Placenta
  • Phenotype
  • Pathology
  • Macrophages
  • Immunohistochemistry