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Endothelial cell diversity revealed by global expression profiling.

Publication ,  Journal Article
Chi, J-T; Chang, HY; Haraldsen, G; Jahnsen, FL; Troyanskaya, OG; Chang, DS; Wang, Z; Rockson, SG; van de Rijn, M; Botstein, D; Brown, PO
Published in: Proc Natl Acad Sci U S A
September 16, 2003

The vascular system is locally specialized to accommodate widely varying blood flow and pressure and the distinct needs of individual tissues. The endothelial cells (ECs) that line the lumens of blood and lymphatic vessels play an integral role in the regional specialization of vascular structure and physiology. However, our understanding of EC diversity is limited. To explore EC specialization on a global scale, we used DNA microarrays to determine the expression profile of 53 cultured ECs. We found that ECs from different blood vessels and microvascular ECs from different tissues have distinct and characteristic gene expression profiles. Pervasive differences in gene expression patterns distinguish the ECs of large vessels from microvascular ECs. We identified groups of genes characteristic of arterial and venous endothelium. Hey2, the human homologue of the zebrafish gene gridlock, was selectively expressed in arterial ECs and induced the expression of several arterial-specific genes. Several genes critical in the establishment of left/right asymmetry were expressed preferentially in venous ECs, suggesting coordination between vascular differentiation and body plan development. Tissue-specific expression patterns in different tissue microvascular ECs suggest they are distinct differentiated cell types that play roles in the local physiology of their respective organs and tissues.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 16, 2003

Volume

100

Issue

19

Start / End Page

10623 / 10628

Location

United States

Related Subject Headings

  • Transcription Factors
  • Humans
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Endothelium, Vascular
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
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Chi, J.-T., Chang, H. Y., Haraldsen, G., Jahnsen, F. L., Troyanskaya, O. G., Chang, D. S., … Brown, P. O. (2003). Endothelial cell diversity revealed by global expression profiling. Proc Natl Acad Sci U S A, 100(19), 10623–10628. https://doi.org/10.1073/pnas.1434429100
Chi, Jen-Tsan, Howard Y. Chang, Guttorm Haraldsen, Frode L. Jahnsen, Olga G. Troyanskaya, Dustin S. Chang, Zhen Wang, et al. “Endothelial cell diversity revealed by global expression profiling.Proc Natl Acad Sci U S A 100, no. 19 (September 16, 2003): 10623–28. https://doi.org/10.1073/pnas.1434429100.
Chi J-T, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, et al. Endothelial cell diversity revealed by global expression profiling. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10623–8.
Chi, Jen-Tsan, et al. “Endothelial cell diversity revealed by global expression profiling.Proc Natl Acad Sci U S A, vol. 100, no. 19, Sept. 2003, pp. 10623–28. Pubmed, doi:10.1073/pnas.1434429100.
Chi J-T, Chang HY, Haraldsen G, Jahnsen FL, Troyanskaya OG, Chang DS, Wang Z, Rockson SG, van de Rijn M, Botstein D, Brown PO. Endothelial cell diversity revealed by global expression profiling. Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10623–10628.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 16, 2003

Volume

100

Issue

19

Start / End Page

10623 / 10628

Location

United States

Related Subject Headings

  • Transcription Factors
  • Humans
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Endothelium, Vascular
  • Cells, Cultured