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Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines.

Publication ,  Journal Article
Ji, J; Chen, X; Leung, SY; Chi, J-TA; Chu, KM; Yuen, ST; Li, R; Chan, ASY; Li, J; Dunphy, N; So, S
Published in: Oncogene
September 19, 2002

Gastric adenocarcinoma is one of the major malignancies worldwide. Gastric cell lines have been widely used as the model to study the genetics, pharmacology and biochemistry of gastric cancers. Here we describe a comprehensive survey of the gene expression profiles of 12 gastric carcinoma cell lines, using cDNA microarray with 43 000 clones. For comparison, we also explored the gene expression patterns of 15 cell lines derived from lymphoid, endothelial, stromal and other epithelial cancers. Expression levels of specific genes were validated through comparison to protein expression by immunohistochemistry using cell block arrays. We found sets of genes whose expression corresponds to the molecular signature of each cell type. In the gastric cancer cell lines, apart from genes that are highly expressed corresponding to their common epithelial origin from the gastrointestinal tract, we found marked heterogeneity among the gene expression patterns of these cell lines. Some of the heterogeneity may reflect their underlying molecular characteristics or specific differentiation program. Two putative gastric carcinoma cell lines were found to be B-cell lymphoma, and another one had no epithelial specific gene expression and hence was of doubtful epithelial origin. These cell lines should no longer be used in gastric carcinoma research. In conclusion, our gene expression database can serve as a powerful resource for the study of gastric cancer using these cell lines.

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Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

September 19, 2002

Volume

21

Issue

42

Start / End Page

6549 / 6556

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Stomach Neoplasms
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Proteins
  • Lymphoma, B-Cell
  • Immunoenzyme Techniques
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Profiling
 

Citation

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ICMJE
MLA
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Ji, J., Chen, X., Leung, S. Y., Chi, J.-T., Chu, K. M., Yuen, S. T., … So, S. (2002). Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines. Oncogene, 21(42), 6549–6556. https://doi.org/10.1038/sj.onc.1205829
Ji, Jiafu, Xin Chen, Suet Yi Leung, Jen-Tsan A. Chi, Kent Man Chu, Siu Tsan Yuen, Rui Li, et al. “Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines.Oncogene 21, no. 42 (September 19, 2002): 6549–56. https://doi.org/10.1038/sj.onc.1205829.
Ji J, Chen X, Leung SY, Chi J-TA, Chu KM, Yuen ST, et al. Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines. Oncogene. 2002 Sep 19;21(42):6549–56.
Ji, Jiafu, et al. “Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines.Oncogene, vol. 21, no. 42, Sept. 2002, pp. 6549–56. Pubmed, doi:10.1038/sj.onc.1205829.
Ji J, Chen X, Leung SY, Chi J-TA, Chu KM, Yuen ST, Li R, Chan ASY, Li J, Dunphy N, So S. Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines. Oncogene. 2002 Sep 19;21(42):6549–6556.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

September 19, 2002

Volume

21

Issue

42

Start / End Page

6549 / 6556

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Stomach Neoplasms
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neoplasm Proteins
  • Lymphoma, B-Cell
  • Immunoenzyme Techniques
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Gene Expression Profiling