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Diagnosis of canine visceral leishmaniasis with a dot-enzyme-linked immunosorbent assay.

Publication ,  Journal Article
Dietze, R; Falqueto, A; Valli, LC; Rodriques, TP; Boulos, M; Corey, R
Published in: Am J Trop Med Hyg
July 1995

The maintenance and transmission of human visceral leishmaniasis in an endemic area usually requires a mammalian reservoir. Though universal reservoir elimination has previously been effective in controlling this disease in countries where the primary reservoir is the dog, selective elimination would be preferable. To guide this selection process, we performed a prospective, single, blind, cohort study evaluating the dot-enzyme-linked immunosorbent assay (dot-ELISA) in 130 canines from both endemic and nonendemic areas. The results were compared with blinded bone marrow aspirate examination and physical assessment of the animals. Using visualization of amastigotes on bone marrow examination as a priori evidence of infection, the dot-ELISA was found to be highly sensitive (97%) and specific (100%). In contrast, the physical evaluation had remarkably low sensitivity and specificity. The dot-ELISA is an excellent test for detection of the canine reservoir of Leishmania. Because it is simple to perform, inexpensive, and highly accurate, it may help control this debilitating illness by facilitating selective canine elimination.

Duke Scholars

Published In

Am J Trop Med Hyg

ISSN

0002-9637

Publication Date

July 1995

Volume

53

Issue

1

Start / End Page

40 / 42

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Single-Blind Method
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Prospective Studies
  • Leishmaniasis, Visceral
  • Leishmania donovani
  • Enzyme-Linked Immunosorbent Assay
  • Dogs
  • Dog Diseases
 

Citation

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Dietze, R., Falqueto, A., Valli, L. C., Rodriques, T. P., Boulos, M., & Corey, R. (1995). Diagnosis of canine visceral leishmaniasis with a dot-enzyme-linked immunosorbent assay. Am J Trop Med Hyg, 53(1), 40–42.
Dietze, R., A. Falqueto, L. C. Valli, T. P. Rodriques, M. Boulos, and R. Corey. “Diagnosis of canine visceral leishmaniasis with a dot-enzyme-linked immunosorbent assay.Am J Trop Med Hyg 53, no. 1 (July 1995): 40–42.
Dietze R, Falqueto A, Valli LC, Rodriques TP, Boulos M, Corey R. Diagnosis of canine visceral leishmaniasis with a dot-enzyme-linked immunosorbent assay. Am J Trop Med Hyg. 1995 Jul;53(1):40–2.
Dietze, R., et al. “Diagnosis of canine visceral leishmaniasis with a dot-enzyme-linked immunosorbent assay.Am J Trop Med Hyg, vol. 53, no. 1, July 1995, pp. 40–42.
Dietze R, Falqueto A, Valli LC, Rodriques TP, Boulos M, Corey R. Diagnosis of canine visceral leishmaniasis with a dot-enzyme-linked immunosorbent assay. Am J Trop Med Hyg. 1995 Jul;53(1):40–42.

Published In

Am J Trop Med Hyg

ISSN

0002-9637

Publication Date

July 1995

Volume

53

Issue

1

Start / End Page

40 / 42

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Single-Blind Method
  • Sensitivity and Specificity
  • Reproducibility of Results
  • Prospective Studies
  • Leishmaniasis, Visceral
  • Leishmania donovani
  • Enzyme-Linked Immunosorbent Assay
  • Dogs
  • Dog Diseases