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Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes.

Publication ,  Journal Article
Kobiler, O; Lipman, Y; Therkelsen, K; Daubechies, I; Enquist, LW
Published in: Nature communications
January 2010

Whether all the infectious herpesvirus particles entering a cell are able to replicate and/or express their genomes is not known. Here, we developed a general method to determine the number of viral genomes expressed in an infected cell. We constructed and analysed fluorophore expression from a recombinant pseudorabies virus (PRV263) carrying a Brainbow cassette (Cre-conditional expression of different fluorophores). Using three isogenic strains derived from PRV263, each expressing a single fluorophore, we analysed the colour composition of cells infected with these three viruses at different multiplicities. We estimate that fewer than seven incoming genomes are expressed per cell. In addition, those templates that are expressed are the genomes selected for replication and packaging into virions. This finite limit on the number of viral genomes that can be expressed is an intrinsic property of the infected cell and may be influenced by viral and cellular factors.

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Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

January 2010

Volume

1

Start / End Page

146

Related Subject Headings

  • Swine
  • Models, Theoretical
  • Microscopy, Fluorescence
  • Herpesviridae
  • Genome, Viral
  • DNA Replication
  • Cell Line
  • Animals
 

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Kobiler, O., Lipman, Y., Therkelsen, K., Daubechies, I., & Enquist, L. W. (2010). Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes. Nature Communications, 1, 146. https://doi.org/10.1038/ncomms1145
Kobiler, Oren, Yaron Lipman, Kate Therkelsen, Ingrid Daubechies, and Lynn W. Enquist. “Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes.Nature Communications 1 (January 2010): 146. https://doi.org/10.1038/ncomms1145.
Kobiler O, Lipman Y, Therkelsen K, Daubechies I, Enquist LW. Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes. Nature communications. 2010 Jan;1:146.
Kobiler, Oren, et al. “Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes.Nature Communications, vol. 1, Jan. 2010, p. 146. Epmc, doi:10.1038/ncomms1145.
Kobiler O, Lipman Y, Therkelsen K, Daubechies I, Enquist LW. Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes. Nature communications. 2010 Jan;1:146.

Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

January 2010

Volume

1

Start / End Page

146

Related Subject Headings

  • Swine
  • Models, Theoretical
  • Microscopy, Fluorescence
  • Herpesviridae
  • Genome, Viral
  • DNA Replication
  • Cell Line
  • Animals