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Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types.

Publication ,  Journal Article
Singla, DK; Hacker, TA; Ma, L; Douglas, PS; Sullivan, R; Lyons, GE; Kamp, TJ
Published in: J Mol Cell Cardiol
January 2006

Initial studies have suggested that transplantation of embryonic stem (ES) cells following myocardial infarction (MI) in animal models is beneficial; however, the mechanism of benefit is largely unknown. The present study investigated the fate of mouse ES cells transplanted post-MI to determine if the ES cells give rise to the range of major cell types present in the native myocardium. MI was produced by coronary artery ligation in C57BL/6 mice. Two different mouse ES cell lines, expressing eGFP and beta-galactosidase, respectively, were tested. Post-MI intramyocardial injection of 3 x 10(4) ES cells was compared to injection of media alone. Histochemistry and immunofluorescence were used to track the transplanted ES cells and identify the resulting cell types. Echocardiography assessed the cardiac size and function in a blinded fashion. Two weeks post-MI, engraftment of the transplanted ES cells was demonstrated by eGFP or beta-galactosidase-positive cells in the infarct region without evidence for tumor formation. Co-immunolabeling demonstrated that the transplanted ES cells had become cardiomyocytes, vascular smooth muscle, and endothelial cells. Echocardiographic analysis showed that ES cell transplantation resulted in reduced post-MI remodeling of the heart and improved cardiac function. In conclusion, transplanted mouse ES cells can regenerate infarcted myocardium in part by becoming cardiomyocytes, vascular smooth muscle, and endothelial cells that result in an improvement in cardiac structure and function. Therefore, ES cells hold promise for myocardial cellular therapy.

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Published In

J Mol Cell Cardiol

DOI

ISSN

0022-2828

Publication Date

January 2006

Volume

40

Issue

1

Start / End Page

195 / 200

Location

England

Related Subject Headings

  • beta-Galactosidase
  • Stem Cell Transplantation
  • Myocytes, Cardiac
  • Myocardium
  • Myocardial Infarction
  • Muscle, Smooth, Vascular
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Female
 

Citation

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Singla, D. K., Hacker, T. A., Ma, L., Douglas, P. S., Sullivan, R., Lyons, G. E., & Kamp, T. J. (2006). Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types. J Mol Cell Cardiol, 40(1), 195–200. https://doi.org/10.1016/j.yjmcc.2005.09.001
Singla, Dinender K., Timothy A. Hacker, Lining Ma, Pamela S. Douglas, Ruth Sullivan, Gary E. Lyons, and Timothy J. Kamp. “Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types.J Mol Cell Cardiol 40, no. 1 (January 2006): 195–200. https://doi.org/10.1016/j.yjmcc.2005.09.001.
Singla DK, Hacker TA, Ma L, Douglas PS, Sullivan R, Lyons GE, et al. Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types. J Mol Cell Cardiol. 2006 Jan;40(1):195–200.
Singla, Dinender K., et al. “Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types.J Mol Cell Cardiol, vol. 40, no. 1, Jan. 2006, pp. 195–200. Pubmed, doi:10.1016/j.yjmcc.2005.09.001.
Singla DK, Hacker TA, Ma L, Douglas PS, Sullivan R, Lyons GE, Kamp TJ. Transplantation of embryonic stem cells into the infarcted mouse heart: formation of multiple cell types. J Mol Cell Cardiol. 2006 Jan;40(1):195–200.
Journal cover image

Published In

J Mol Cell Cardiol

DOI

ISSN

0022-2828

Publication Date

January 2006

Volume

40

Issue

1

Start / End Page

195 / 200

Location

England

Related Subject Headings

  • beta-Galactosidase
  • Stem Cell Transplantation
  • Myocytes, Cardiac
  • Myocardium
  • Myocardial Infarction
  • Muscle, Smooth, Vascular
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Female