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Chronic growth hormone treatment in normal rats reduces post-prandial skeletal muscle plasma membrane GLUT1 content, but not glucose transport or GLUT4 expression and localization.

Publication ,  Journal Article
Napoli, R; Cittadini, A; Chow, JC; Hirshman, MF; Smith, RJ; Douglas, PS; Horton, ES
Published in: Biochem J
May 1, 1996

Whether skeletal muscle glucose transport system is impaired in the basal, post-prandial state during chronic growth hormone treatment is unknown. The current study was designed to determine whether 4 weeks of human growth hormone (hGH) treatment (3.5 mg/kg per day) would impair glucose transport and/or the number of glucose transporters in plasma membrane vesicles isolated from hindlimb skeletal muscle of Sprague-Dawley rats under basal, post-prandial conditions. hGH treatment was shown to have no effect on glucose influx (Vmax or K(m)) determined under equilibrium exchange conditions in isolated plasma membrane vesicles. Plasma membrane glucose transporter number (Ro) measured by cytochalasin B binding was also unchanged by hGH treatment. Consequently, glucose transporter turnover number (Vmax/Ro), a measure of average glucose transporter intrinsic activity, was similar in hGH-treated and control rats. hGH did not change GLUT4 protein content in whole muscle or in the plasma membrane, and muscle content of GLUT4 mRNA also was unchanged. In contrast, GLUT1 protein content in the plasma membrane fraction was significantly reduced by hGH treatment. This was associated with a modest, although not significant, decrease in muscle content of GLUT1 mRNA. In conclusion, high-dose hGH treatment for 4 weeks did not alter post-prandial skeletal muscle glucose transport activity. Neither the muscle level nor the intracellular localization of GLUT4 was changed by the hormone treatment. On the contrary, the basal post-prandial level of GLUT1 in the plasma membrane was reduced by hGH. The mRNA data suggest that this reduction might result from a decrease in the synthesis of GLUT1.

Duke Scholars

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

May 1, 1996

Volume

315 ( Pt 3)

Issue

Pt 3

Start / End Page

959 / 963

Location

England

Related Subject Headings

  • Tissue Distribution
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Muscle, Skeletal
  • Muscle Proteins
  • Monosaccharide Transport Proteins
  • Kinetics
  • Humans
  • Growth Hormone
 

Citation

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Napoli, R., Cittadini, A., Chow, J. C., Hirshman, M. F., Smith, R. J., Douglas, P. S., & Horton, E. S. (1996). Chronic growth hormone treatment in normal rats reduces post-prandial skeletal muscle plasma membrane GLUT1 content, but not glucose transport or GLUT4 expression and localization. Biochem J, 315 ( Pt 3)(Pt 3), 959–963. https://doi.org/10.1042/bj3150959
Napoli, R., A. Cittadini, J. C. Chow, M. F. Hirshman, R. J. Smith, P. S. Douglas, and E. S. Horton. “Chronic growth hormone treatment in normal rats reduces post-prandial skeletal muscle plasma membrane GLUT1 content, but not glucose transport or GLUT4 expression and localization.Biochem J 315 ( Pt 3), no. Pt 3 (May 1, 1996): 959–63. https://doi.org/10.1042/bj3150959.
Napoli R, Cittadini A, Chow JC, Hirshman MF, Smith RJ, Douglas PS, et al. Chronic growth hormone treatment in normal rats reduces post-prandial skeletal muscle plasma membrane GLUT1 content, but not glucose transport or GLUT4 expression and localization. Biochem J. 1996 May 1;315 ( Pt 3)(Pt 3):959–63.
Napoli R, Cittadini A, Chow JC, Hirshman MF, Smith RJ, Douglas PS, Horton ES. Chronic growth hormone treatment in normal rats reduces post-prandial skeletal muscle plasma membrane GLUT1 content, but not glucose transport or GLUT4 expression and localization. Biochem J. 1996 May 1;315 ( Pt 3)(Pt 3):959–963.
Journal cover image

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

May 1, 1996

Volume

315 ( Pt 3)

Issue

Pt 3

Start / End Page

959 / 963

Location

England

Related Subject Headings

  • Tissue Distribution
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Muscle, Skeletal
  • Muscle Proteins
  • Monosaccharide Transport Proteins
  • Kinetics
  • Humans
  • Growth Hormone