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Kinesin: switch I & II and the motor mechanism.

Publication ,  Journal Article
Kull, FJ; Endow, SA
Published in: J Cell Sci
January 1, 2002

New crystal structures of the kinesin motors differ from previously described motor-ADP atomic models, showing striking changes both in the switch I region near the nucleotide-binding cleft and in the switch II or 'relay' helix at the filament-binding face of the motor. The switch I region, present as a short helix flanked by two loops in previous motor-ADP structures, rearranges into a pseudo-beta-hairpin or is completely disordered with melted helices to either side of the disordered switch I loop. The relay helix undergoes a rotational movement coupled to a translation that differs from the piston-like movement of the relay helix observed in myosin. The changes observed in the crystal structures are interpreted to represent structural transitions that occur in the kinesin motors during the ATP hydrolysis cycle. The movements of switch I residues disrupt the water-mediated coordination of the bound Mg2+, which could result in loss of Mg2+ and ADP, raising the intriguing possibility that disruption of the switch I region leads to release of nucleotide by the kinesins. None of the new structures is a true motor-ATP state, however, probably because conformational changes at the active site of the kinesins require interactions with microtubules to stabilize the movements.

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Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

January 1, 2002

Volume

115

Issue

Pt 1

Start / End Page

15 / 23

Location

England

Related Subject Headings

  • Structure-Activity Relationship
  • Protein Structure, Secondary
  • Protein Conformation
  • Nucleotides
  • Myosins
  • Molecular Motor Proteins
  • Models, Molecular
  • Magnesium
  • Kinesins
  • Humans
 

Citation

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Kull, F. J., & Endow, S. A. (2002). Kinesin: switch I & II and the motor mechanism. J Cell Sci, 115(Pt 1), 15–23. https://doi.org/10.1242/jcs.115.1.15
Kull, F Jon, and Sharyn A. Endow. “Kinesin: switch I & II and the motor mechanism.J Cell Sci 115, no. Pt 1 (January 1, 2002): 15–23. https://doi.org/10.1242/jcs.115.1.15.
Kull FJ, Endow SA. Kinesin: switch I & II and the motor mechanism. J Cell Sci. 2002 Jan 1;115(Pt 1):15–23.
Kull, F. Jon, and Sharyn A. Endow. “Kinesin: switch I & II and the motor mechanism.J Cell Sci, vol. 115, no. Pt 1, Jan. 2002, pp. 15–23. Pubmed, doi:10.1242/jcs.115.1.15.
Kull FJ, Endow SA. Kinesin: switch I & II and the motor mechanism. J Cell Sci. 2002 Jan 1;115(Pt 1):15–23.
Journal cover image

Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

January 1, 2002

Volume

115

Issue

Pt 1

Start / End Page

15 / 23

Location

England

Related Subject Headings

  • Structure-Activity Relationship
  • Protein Structure, Secondary
  • Protein Conformation
  • Nucleotides
  • Myosins
  • Molecular Motor Proteins
  • Models, Molecular
  • Magnesium
  • Kinesins
  • Humans