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The Drosophila claret segregation protein is a minus-end directed motor molecule.

Publication ,  Journal Article
Walker, RA; Salmon, ED; Endow, SA
Published in: Nature
October 25, 1990

A product encoded at the claret locus in Drosophila is needed for normal chromosome segregation in meiosis in females and in early mitotic divisions of the embryo. The predicted amino-acid sequence of the segregation protein was shown recently to be strikingly similar to Drosophila kinesin heavy chain. We have expressed the claret segregation protein in bacteria and have found that the bacterially expressed protein has motor activity in vitro with several novel features. The claret motor is slow (4 microns min-1), unlike either kinesin or dyneins. It has the directionality, the ability to generate torque and the sensitivity to inhibitors reported previously for dyneins. The finding of minus-end directed motor activity for a protein with sequence similarity to kinesin suggests that the dynein and kinesin motor domains are ancestrally related. The minus-end directed motor activity of the claret motor is consistent with a role for this protein in producing chromosome movement along spindle microtubules during prometaphase and/or anaphase.

Duke Scholars

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

October 25, 1990

Volume

347

Issue

6295

Start / End Page

780 / 782

Location

England

Related Subject Headings

  • Tubulin
  • Recombinant Proteins
  • Movement
  • Mitosis
  • Microtubules
  • Microtubule-Associated Proteins
  • Microscopy, Fluorescence
  • Meiosis
  • Kinetics
  • Kinesins
 

Citation

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MLA
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Walker, R. A., Salmon, E. D., & Endow, S. A. (1990). The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature, 347(6295), 780–782. https://doi.org/10.1038/347780a0
Walker, R. A., E. D. Salmon, and S. A. Endow. “The Drosophila claret segregation protein is a minus-end directed motor molecule.Nature 347, no. 6295 (October 25, 1990): 780–82. https://doi.org/10.1038/347780a0.
Walker RA, Salmon ED, Endow SA. The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature. 1990 Oct 25;347(6295):780–2.
Walker, R. A., et al. “The Drosophila claret segregation protein is a minus-end directed motor molecule.Nature, vol. 347, no. 6295, Oct. 1990, pp. 780–82. Pubmed, doi:10.1038/347780a0.
Walker RA, Salmon ED, Endow SA. The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature. 1990 Oct 25;347(6295):780–782.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

October 25, 1990

Volume

347

Issue

6295

Start / End Page

780 / 782

Location

England

Related Subject Headings

  • Tubulin
  • Recombinant Proteins
  • Movement
  • Mitosis
  • Microtubules
  • Microtubule-Associated Proteins
  • Microscopy, Fluorescence
  • Meiosis
  • Kinetics
  • Kinesins