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Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC.

Publication ,  Journal Article
Kucukdereli, H; Allen, NJ; Lee, AT; Feng, A; Ozlu, MI; Conatser, LM; Chakraborty, C; Workman, G; Weaver, M; Sage, EH; Barres, BA; Eroglu, C
Published in: Proc Natl Acad Sci U S A
August 9, 2011

Astrocytes regulate synaptic connectivity in the CNS through secreted signals. Here we identified two astrocyte-secreted proteins, hevin and SPARC, as regulators of excitatory synaptogenesis in vitro and in vivo. Hevin induces the formation of synapses between cultured rat retinal ganglion cells. SPARC is not synaptogenic, but specifically antagonizes synaptogenic function of hevin. Hevin and SPARC are expressed by astrocytes in the superior colliculus, the synaptic target of retinal ganglion cells, concurrent with the excitatory synaptogenesis. Hevin-null mice had fewer excitatory synapses; conversely, SPARC-null mice had increased synaptic connections in the superior colliculus. Furthermore, we found that hevin is required for the structural maturation of the retinocollicular synapses. These results identify hevin as a positive and SPARC as a negative regulator of synapse formation and signify that, through regulation of relative levels of hevin and SPARC, astrocytes might control the formation, maturation, and plasticity of synapses in vivo.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

August 9, 2011

Volume

108

Issue

32

Start / End Page

E440 / E449

Location

United States

Related Subject Headings

  • Synapses
  • Superior Colliculi
  • Retinal Ganglion Cells
  • Rats, Sprague-Dawley
  • Rats
  • Protein Structure, Tertiary
  • Osteonectin
  • Neurogenesis
  • Mice
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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Kucukdereli, H., Allen, N. J., Lee, A. T., Feng, A., Ozlu, M. I., Conatser, L. M., … Eroglu, C. (2011). Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC. Proc Natl Acad Sci U S A, 108(32), E440–E449. https://doi.org/10.1073/pnas.1104977108
Kucukdereli, Hakan, Nicola J. Allen, Anthony T. Lee, Ava Feng, M Ilcim Ozlu, Laura M. Conatser, Chandrani Chakraborty, et al. “Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC.Proc Natl Acad Sci U S A 108, no. 32 (August 9, 2011): E440–49. https://doi.org/10.1073/pnas.1104977108.
Kucukdereli H, Allen NJ, Lee AT, Feng A, Ozlu MI, Conatser LM, et al. Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC. Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):E440–9.
Kucukdereli, Hakan, et al. “Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC.Proc Natl Acad Sci U S A, vol. 108, no. 32, Aug. 2011, pp. E440–49. Pubmed, doi:10.1073/pnas.1104977108.
Kucukdereli H, Allen NJ, Lee AT, Feng A, Ozlu MI, Conatser LM, Chakraborty C, Workman G, Weaver M, Sage EH, Barres BA, Eroglu C. Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC. Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):E440–E449.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

August 9, 2011

Volume

108

Issue

32

Start / End Page

E440 / E449

Location

United States

Related Subject Headings

  • Synapses
  • Superior Colliculi
  • Retinal Ganglion Cells
  • Rats, Sprague-Dawley
  • Rats
  • Protein Structure, Tertiary
  • Osteonectin
  • Neurogenesis
  • Mice
  • Humans