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Detection and partial characterization of human thymus-leukemia antigens.

Publication ,  Journal Article
Dowell, BL; Falletta, JM; Moore, JO; Metzgar, RS
Published in: J Natl Cancer Inst
October 1980

Two monkey antisera against human thymocytes after absorption with human erythrocytes and peripheral blood leukocytes were shown to detect human thymus-leukemia (HTL)-like antigens. These sera were cytotoxic for thymocytes (> 90% lysis at a 1:10 dilution) but were nonreactive with enriched peripheral blood T- and B-lymphocytes or with cells from myeloid or B-cell lymphoid leukemias. Most (16/17) sheep erythrocyte rosette-forming acute lymphoblastic leukemia (ALL) cells reacted with these sera. Cells from patients with T-cell chronic lymphocytic leukemia, lymphoblastic lymphoma (LBL), and thymoma were also positive. Three of 4 T-cell lymphoblastoid lines derived from ALL patients reacted with these sera. Absorption of the sera with MOLT-4F cells, thymocytes, or LBL cells removed the reactivity against all types of cells tested. However, sera absorbed with the T-cell line HSB remained cytotoxic for thymocytes, MOLT-4F, and most (6/9) T-cell cancers tested. The peripheral blood cell-absorbed sera precipitated a molecule with an apparent molecular weight of 48,000 from lactoperoxidase-labeled thymocytes but not from similarly labeled peripheral blood lymphocytes. The ability of the sera to precipitate this antigen was decreased by absorption with thymocytes, MOLT-4, or LBL cells but not by absorption with HSB, SB, or non-T, non-B ALL cells. Sequential precipitation studies suggested that the HTL antigen was not associated with beta 2 microglobulin.

Duke Scholars

Published In

J Natl Cancer Inst

DOI

ISSN

0027-8874

Publication Date

October 1980

Volume

65

Issue

4

Start / End Page

691 / 701

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Oncology & Carcinogenesis
  • Middle Aged
  • Leukemia, Lymphoid
  • Leukemia
  • Immune Sera
  • Humans
  • Complement System Proteins
  • Child, Preschool
 

Citation

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Chicago
ICMJE
MLA
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Dowell, B. L., Falletta, J. M., Moore, J. O., & Metzgar, R. S. (1980). Detection and partial characterization of human thymus-leukemia antigens. J Natl Cancer Inst, 65(4), 691–701. https://doi.org/10.1093/jnci/65.4.691
Dowell, B. L., J. M. Falletta, J. O. Moore, and R. S. Metzgar. “Detection and partial characterization of human thymus-leukemia antigens.J Natl Cancer Inst 65, no. 4 (October 1980): 691–701. https://doi.org/10.1093/jnci/65.4.691.
Dowell BL, Falletta JM, Moore JO, Metzgar RS. Detection and partial characterization of human thymus-leukemia antigens. J Natl Cancer Inst. 1980 Oct;65(4):691–701.
Dowell, B. L., et al. “Detection and partial characterization of human thymus-leukemia antigens.J Natl Cancer Inst, vol. 65, no. 4, Oct. 1980, pp. 691–701. Pubmed, doi:10.1093/jnci/65.4.691.
Dowell BL, Falletta JM, Moore JO, Metzgar RS. Detection and partial characterization of human thymus-leukemia antigens. J Natl Cancer Inst. 1980 Oct;65(4):691–701.
Journal cover image

Published In

J Natl Cancer Inst

DOI

ISSN

0027-8874

Publication Date

October 1980

Volume

65

Issue

4

Start / End Page

691 / 701

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Oncology & Carcinogenesis
  • Middle Aged
  • Leukemia, Lymphoid
  • Leukemia
  • Immune Sera
  • Humans
  • Complement System Proteins
  • Child, Preschool