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Characteristics of cultured human renal cortical epithelia.

Publication ,  Journal Article
States, B; Foreman, J; Lee, J; Segal, S
Published in: Biochem Med Metab Biol
October 1986

Nine human kidney epithelial cell lines, isolated from small biopsied material and from whole kidney, were propagated in both a hormonally defined medium and a medium supplemented with serum. At confluency, hemicysts or domes, typical of cultured epithelial cells, were formed by these cells. Monolayers had junctional complexes between cells and the presence of numerous microvilli on the cell surface. Parathyroid hormone markedly stimulated these cells to produce cyclic AMP. They also contained high levels of gamma-glutamyltranspeptidase, leucine aminopeptidase, and maltase, enzymes that are associated with the brush-border membrane of the proximal tubule. The cultured cells demonstrated the ability to transport amino acids and alpha-methylglucoside, a substrate actively transported only by the proximal tubule in the kidney. Based on these findings, the cultured cells reflected a number of characteristics associated with the proximal tubule. These renal epithelial cell lines may provide a useful model for studying various aspects of human renal physiology and biochemistry.

Duke Scholars

Published In

Biochem Med Metab Biol

DOI

ISSN

0885-4505

Publication Date

October 1986

Volume

36

Issue

2

Start / End Page

151 / 161

Location

United States

Related Subject Headings

  • Microvilli
  • Methylglucosides
  • Kidney Cortex
  • Humans
  • Hormones
  • Epithelium
  • Cyclic AMP
  • Child
  • Cell Line
  • Biological Transport, Active
 

Citation

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States, B., Foreman, J., Lee, J., & Segal, S. (1986). Characteristics of cultured human renal cortical epithelia. Biochem Med Metab Biol, 36(2), 151–161. https://doi.org/10.1016/0885-4505(86)90119-2
States, B., J. Foreman, J. Lee, and S. Segal. “Characteristics of cultured human renal cortical epithelia.Biochem Med Metab Biol 36, no. 2 (October 1986): 151–61. https://doi.org/10.1016/0885-4505(86)90119-2.
States B, Foreman J, Lee J, Segal S. Characteristics of cultured human renal cortical epithelia. Biochem Med Metab Biol. 1986 Oct;36(2):151–61.
States, B., et al. “Characteristics of cultured human renal cortical epithelia.Biochem Med Metab Biol, vol. 36, no. 2, Oct. 1986, pp. 151–61. Pubmed, doi:10.1016/0885-4505(86)90119-2.
States B, Foreman J, Lee J, Segal S. Characteristics of cultured human renal cortical epithelia. Biochem Med Metab Biol. 1986 Oct;36(2):151–161.

Published In

Biochem Med Metab Biol

DOI

ISSN

0885-4505

Publication Date

October 1986

Volume

36

Issue

2

Start / End Page

151 / 161

Location

United States

Related Subject Headings

  • Microvilli
  • Methylglucosides
  • Kidney Cortex
  • Humans
  • Hormones
  • Epithelium
  • Cyclic AMP
  • Child
  • Cell Line
  • Biological Transport, Active