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Impacts of organic carbon availability and recipient bacteria characteristics on the potential for TOL plasmid genetic bioaugmentation in soil slurries.

Publication ,  Journal Article
Ikuma, K; Holzem, RM; Gunsch, CK
Published in: Chemosphere
September 2012

The effectiveness of genetic bioaugmentation relies on efficient plasmid transfer between donor and recipient cells as well as the plasmid's phenotype in the recipient cell. In the present study, the effects of varying organic carbon substrates, initial recipient-to-donor cell density ratios, and mixtures of known recipient bacterial strains on the conjugation and function of a TOL plasmid were tested in sterile soil slurry batch reactors. The presence of soil organic carbon was sufficient in ensuring TOL plasmid transconjugant occurrence (up to 2.1±0.5%) for most recipient strains in soil slurry batch mating experiments. The addition of glucose had limited effects on transconjugant occurrence; however, glucose amendment increased the specific toluene degradation rates of some Enterobacteriaceae transconjugants in soil slurry. Initial cell density ratios and mixtures of recipient strains had smaller impacts on plasmid conjugation and resulting phenotype functionality. These observations suggest that genetic bioaugmentation may be improved by minimal altering of environmental conditions.

Duke Scholars

Published In

Chemosphere

DOI

EISSN

1879-1298

ISSN

0045-6535

Publication Date

September 2012

Volume

89

Issue

2

Start / End Page

158 / 163

Related Subject Headings

  • Toluene
  • Soil Microbiology
  • Plasmids
  • Meteorology & Atmospheric Sciences
  • Glucose
  • Genetic Engineering
  • Environmental Sciences
  • Environmental Pollutants
  • Bioreactors
  • Biodegradation, Environmental
 

Citation

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Ikuma, K., Holzem, R. M., & Gunsch, C. K. (2012). Impacts of organic carbon availability and recipient bacteria characteristics on the potential for TOL plasmid genetic bioaugmentation in soil slurries. Chemosphere, 89(2), 158–163. https://doi.org/10.1016/j.chemosphere.2012.05.086
Ikuma, Kaoru, Ryan M. Holzem, and Claudia K. Gunsch. “Impacts of organic carbon availability and recipient bacteria characteristics on the potential for TOL plasmid genetic bioaugmentation in soil slurries.Chemosphere 89, no. 2 (September 2012): 158–63. https://doi.org/10.1016/j.chemosphere.2012.05.086.
Ikuma, Kaoru, et al. “Impacts of organic carbon availability and recipient bacteria characteristics on the potential for TOL plasmid genetic bioaugmentation in soil slurries.Chemosphere, vol. 89, no. 2, Sept. 2012, pp. 158–63. Epmc, doi:10.1016/j.chemosphere.2012.05.086.
Journal cover image

Published In

Chemosphere

DOI

EISSN

1879-1298

ISSN

0045-6535

Publication Date

September 2012

Volume

89

Issue

2

Start / End Page

158 / 163

Related Subject Headings

  • Toluene
  • Soil Microbiology
  • Plasmids
  • Meteorology & Atmospheric Sciences
  • Glucose
  • Genetic Engineering
  • Environmental Sciences
  • Environmental Pollutants
  • Bioreactors
  • Biodegradation, Environmental