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Salt-split human skin substrate for the immunofluorescent screening of serum from patients with cicatricial pemphigoid and a new method of immunoprecipitation with IgA antibodies

Publication ,  Journal Article
Sarret, Y; Hall, R; Cobo, LM; Thivolet, J; Patton, DL; Woodley, DT
Published in: Journal of the American Academy of Dermatology
1991

In patients with cicatricial pemphigoid, immunoglobulins (usually IgG) and complement are deposited within the dermoepidermal junction and are detected by direct immunofluorescent staining of perilesional mucous membrane and/or skin with fluorescein-labeled antibodies to human immunoglobulins. Although rare, some patients also have circulating low-titer, anti-basement membrane zone autoantibodies. In this study, we report 11 patients with the clinical, histologic, and immunologic criteria for cicatricial pemphigoid who had circulating anti-BMZ autoantibodies as demonstrated by positive indirect staining of a normal human skin that had been fractured through the dermoepidermal junction by prolonged incubation in a cold, 1 mol/L sodium chloride solution. On this salt-split human skin substrate, 9 of the 11 patients (82%) had autoantibodies that bound to the epidermal roof, one serum stained only the dermal floor, and one serum stained both sides of the separation. The predominant class of immunoglobulin in the patients' sera that bound to the substrate was IgA; IgA was the single immunoglobulin in 55% and was associated with IgG in 18%. IgG was the only immunoglobulin detected in 27% of the cases. No specific protein was detected by either Western immunoblot or a new IgA immunoprecipitation procedure.

Duke Scholars

Published In

Journal of the American Academy of Dermatology

ISSN

0190-9622

Publication Date

1991

Volume

24

Issue

6 I

Start / End Page

952 / 958

Related Subject Headings

  • Dermatology & Venereal Diseases
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
 

Citation

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Sarret, Y., Hall, R., Cobo, L. M., Thivolet, J., Patton, D. L., & Woodley, D. T. (1991). Salt-split human skin substrate for the immunofluorescent screening of serum from patients with cicatricial pemphigoid and a new method of immunoprecipitation with IgA antibodies. Journal of the American Academy of Dermatology, 24(6 I), 952–958.
Sarret, Y., R. Hall, L. M. Cobo, J. Thivolet, D. L. Patton, and D. T. Woodley. “Salt-split human skin substrate for the immunofluorescent screening of serum from patients with cicatricial pemphigoid and a new method of immunoprecipitation with IgA antibodies.” Journal of the American Academy of Dermatology 24, no. 6 I (1991): 952–58.
Sarret Y, Hall R, Cobo LM, Thivolet J, Patton DL, Woodley DT. Salt-split human skin substrate for the immunofluorescent screening of serum from patients with cicatricial pemphigoid and a new method of immunoprecipitation with IgA antibodies. Journal of the American Academy of Dermatology. 1991;24(6 I):952–8.
Sarret Y, Hall R, Cobo LM, Thivolet J, Patton DL, Woodley DT. Salt-split human skin substrate for the immunofluorescent screening of serum from patients with cicatricial pemphigoid and a new method of immunoprecipitation with IgA antibodies. Journal of the American Academy of Dermatology. 1991;24(6 I):952–958.
Journal cover image

Published In

Journal of the American Academy of Dermatology

ISSN

0190-9622

Publication Date

1991

Volume

24

Issue

6 I

Start / End Page

952 / 958

Related Subject Headings

  • Dermatology & Venereal Diseases
  • 3202 Clinical sciences
  • 1103 Clinical Sciences