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The transneuronal transport of horseradish peroxidase in the visual system of the frog, Rana pipiens.

Publication ,  Journal Article
Hughes, TE; Hall, WC
Published in: Neuroscience
February 1986

During the course of experiments designed to study synaptic relationships between the terminals of retinal axons and the various cell populations in the optic tectum of the frog, Rana pipiens, we found that neurons in many of the retinorecipient nuclei, including the tectum, are labeled transneuronally following injections of horseradish peroxidase into the optic nerve. In the optic tectum, particular cell groups are labeled to the extent that their dendrites as well as their somas are filled with reaction product while other cell types, which, on the basis of the location of their somas or dendrites, seem equally likely to receive direct retinal projections, remain free of label. Electron microscopic investigation of the optic tectum reveals that the label is confined to pre- and postsynaptic processes. These results suggest that the transneuronal transport depends on a transfer of horseradish peroxidase from presynaptic terminals to postsynaptic cells rather than on a widespread diffusion of the enzyme through the neuropil followed by a selective uptake by particular cell groups. These results also suggest that only some of the tectal cell groups which receive direct retinal projections may be transneuronally labeled. The transneuronal transport of horseradish peroxidase is useful since it reveals the morphology as well as the location of at least some of the retinorecipient cells. Moreover, the robust nature of this phenomenon makes the frog a good choice for future studies of the mechanism of transneuronal transport.

Duke Scholars

Published In

Neuroscience

DOI

ISSN

0306-4522

Publication Date

February 1986

Volume

17

Issue

2

Start / End Page

507 / 518

Location

United States

Related Subject Headings

  • Visual Pathways
  • Superior Colliculi
  • Retina
  • Rana pipiens
  • Peroxidases
  • Neurons
  • Neurology & Neurosurgery
  • Microscopy, Electron
  • Male
  • Horseradish Peroxidase
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hughes, T. E., & Hall, W. C. (1986). The transneuronal transport of horseradish peroxidase in the visual system of the frog, Rana pipiens. Neuroscience, 17(2), 507–518. https://doi.org/10.1016/0306-4522(86)90263-0
Hughes, T. E., and W. C. Hall. “The transneuronal transport of horseradish peroxidase in the visual system of the frog, Rana pipiens.Neuroscience 17, no. 2 (February 1986): 507–18. https://doi.org/10.1016/0306-4522(86)90263-0.
Hughes, T. E., and W. C. Hall. “The transneuronal transport of horseradish peroxidase in the visual system of the frog, Rana pipiens.Neuroscience, vol. 17, no. 2, Feb. 1986, pp. 507–18. Pubmed, doi:10.1016/0306-4522(86)90263-0.
Journal cover image

Published In

Neuroscience

DOI

ISSN

0306-4522

Publication Date

February 1986

Volume

17

Issue

2

Start / End Page

507 / 518

Location

United States

Related Subject Headings

  • Visual Pathways
  • Superior Colliculi
  • Retina
  • Rana pipiens
  • Peroxidases
  • Neurons
  • Neurology & Neurosurgery
  • Microscopy, Electron
  • Male
  • Horseradish Peroxidase