Cellular composition and ultrastructure of hepatic neoplasms induced by diethylnitrosamine in Oryzias latipes

In vivo exposure of young Oryzias latipes to the carcinogen, diethylnitrosamine (DENA), results in tumor formation after a brief latent period.^1,2 During serial analysis of cytologic changes accompanying, neoplastic progression,³ we reported light microscopic alterations and appearance of enzyme-altered foci. In addition, we have shown hepatocellular neoplasms containing basophilic hepatocytes. These were arranged as thickened tubules, solid round cellular masses and elongated, triangular-shaped cells forming irregular extensions (poorly differentiated tumor). Common cytologic features of tumor cells and cells of early altered foci (enzyme and tinctorial) suggested rapid neoplastic transformation of hepatocytes. The purpose of this study was to examine the ultrastructure of alterations occurring with development of hepatic neoplasms in O. latipes exposed to a tumorigenic concentration of DENA. © 1988.

Duke Scholars

Author David E. Hinton Environmental Natural Science