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Depletion of energy reserve via the creatine kinase reaction during the evolution of heart failure in cardiomyopathic hamsters.

Publication ,  Journal Article
Tian, R; Nascimben, L; Kaddurah-Daouk, R; Ingwall, JS
Published in: J Mol Cell Cardiol
April 1996

To study the contribution of myocardial energy reserve to the deterioration of cardiac function during the development of heart failure, we defined energy reserve via the creatine kinase (CK) reaction and the isovolumic contractile performance in hearts of cardiomyopathic hamsters at the ages of 1.5, 4, 17, 30 and 43 weeks and in age-matched normal hamsters. Energy reserve via the CK reaction was estimated by the product of total CK activity and the content of total creatine in the heart. Isovolumic contractile performance was measured as rate pressure product (RPP, 10(3) mmHg/min) in isolated hearts. Contractile reserve was assessed as the increase of RPP elicited by high calcium stimulation. Compared to the controls, decreases in total CK activity and content of total creatine were observed in hearts of 17-, 30- and 43-week-old cardiomyopathic hamsters. These changes were not observed in the skeletal muscle. Although the decrease of baseline RPP first occurred at the age of 30 weeks (11.5 +/- 0.9 v 20.5 +/- 0.8, P < 0.05), the contractile reserve was already reduced at the age of 17 weeks (9.9 +/- 1.3 v 23.6 +/- 1.9, P < 0.05). A linear relationship was found between the energy reserve via creatine kinase reaction and the contractile reserve of the heart (r2 = 0.85). Furthermore, concomitant decreases in the CK reaction velocity and the contractile reserve were observed in cardiomyopathic hearts, suggesting that depletion of energy reserve may contribute to the development of heart failure.

Duke Scholars

Published In

J Mol Cell Cardiol

DOI

ISSN

0022-2828

Publication Date

April 1996

Volume

28

Issue

4

Start / End Page

755 / 765

Location

England

Related Subject Headings

  • Myocardial Contraction
  • Mesocricetus
  • Male
  • Energy Metabolism
  • Cricetinae
  • Creatine Kinase
  • Cardiovascular System & Hematology
  • Cardiomyopathies
  • Cardiac Output, Low
  • Calcium
 

Citation

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Tian, R., Nascimben, L., Kaddurah-Daouk, R., & Ingwall, J. S. (1996). Depletion of energy reserve via the creatine kinase reaction during the evolution of heart failure in cardiomyopathic hamsters. J Mol Cell Cardiol, 28(4), 755–765. https://doi.org/10.1006/jmcc.1996.0070
Tian, R., L. Nascimben, R. Kaddurah-Daouk, and J. S. Ingwall. “Depletion of energy reserve via the creatine kinase reaction during the evolution of heart failure in cardiomyopathic hamsters.J Mol Cell Cardiol 28, no. 4 (April 1996): 755–65. https://doi.org/10.1006/jmcc.1996.0070.
Tian R, Nascimben L, Kaddurah-Daouk R, Ingwall JS. Depletion of energy reserve via the creatine kinase reaction during the evolution of heart failure in cardiomyopathic hamsters. J Mol Cell Cardiol. 1996 Apr;28(4):755–65.
Tian, R., et al. “Depletion of energy reserve via the creatine kinase reaction during the evolution of heart failure in cardiomyopathic hamsters.J Mol Cell Cardiol, vol. 28, no. 4, Apr. 1996, pp. 755–65. Pubmed, doi:10.1006/jmcc.1996.0070.
Tian R, Nascimben L, Kaddurah-Daouk R, Ingwall JS. Depletion of energy reserve via the creatine kinase reaction during the evolution of heart failure in cardiomyopathic hamsters. J Mol Cell Cardiol. 1996 Apr;28(4):755–765.
Journal cover image

Published In

J Mol Cell Cardiol

DOI

ISSN

0022-2828

Publication Date

April 1996

Volume

28

Issue

4

Start / End Page

755 / 765

Location

England

Related Subject Headings

  • Myocardial Contraction
  • Mesocricetus
  • Male
  • Energy Metabolism
  • Cricetinae
  • Creatine Kinase
  • Cardiovascular System & Hematology
  • Cardiomyopathies
  • Cardiac Output, Low
  • Calcium