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Reaper-induced dissociation of a Scythe-sequestered cytochrome c-releasing activity.

Publication ,  Journal Article
Thress, K; Evans, EK; Kornbluth, S
Published in: The EMBO journal
October 1999

Reaper is a potent apoptotic inducer critical for programmed cell death in the fly Drosophila melanogaster. While Reaper homologs from other species have not yet been reported, ectopic expression of Reaper in cells of vertebrate origin can also trigger apoptosis, suggesting that Reaper-responsive pathways are likely to be conserved. We recently reported that Reaper-induced mitochondrial cytochrome c release and caspase activation in a cell-free extract of Xenopus eggs requires the presence of a 150 kDa Reaper-binding protein, Scythe. We now show that Reaper binding to Scythe causes Scythe to release a sequestered apoptotic inducer. Upon release, the Scythe-sequestered factor(s) is sufficient to induce cytochrome c release from purified mitochondria. Moreover, addition of excess Scythe to egg extracts impedes Reaper-induced apoptosis, most likely through rebinding of the released factors. In addition to Reaper, Scythe binds two other Drosophila apoptotic regulators: Grim and Hid. Surprisingly, however, the region of Reaper which is detectably homologous to Grim and Hid is dispensable for Scythe binding.

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Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

October 1999

Volume

18

Issue

20

Start / End Page

5486 / 5493

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Recombinant Proteins
  • Peptides
  • Oocytes
  • Molecular Chaperones
  • Mitochondria
  • In Vitro Techniques
  • Genetic Variation
  • Female
 

Citation

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Thress, K., Evans, E. K., & Kornbluth, S. (1999). Reaper-induced dissociation of a Scythe-sequestered cytochrome c-releasing activity. The EMBO Journal, 18(20), 5486–5493. https://doi.org/10.1093/emboj/18.20.5486
Thress, K., E. K. Evans, and S. Kornbluth. “Reaper-induced dissociation of a Scythe-sequestered cytochrome c-releasing activity.The EMBO Journal 18, no. 20 (October 1999): 5486–93. https://doi.org/10.1093/emboj/18.20.5486.
Thress K, Evans EK, Kornbluth S. Reaper-induced dissociation of a Scythe-sequestered cytochrome c-releasing activity. The EMBO journal. 1999 Oct;18(20):5486–93.
Thress, K., et al. “Reaper-induced dissociation of a Scythe-sequestered cytochrome c-releasing activity.The EMBO Journal, vol. 18, no. 20, Oct. 1999, pp. 5486–93. Epmc, doi:10.1093/emboj/18.20.5486.
Thress K, Evans EK, Kornbluth S. Reaper-induced dissociation of a Scythe-sequestered cytochrome c-releasing activity. The EMBO journal. 1999 Oct;18(20):5486–5493.

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

October 1999

Volume

18

Issue

20

Start / End Page

5486 / 5493

Related Subject Headings

  • Xenopus Proteins
  • Xenopus
  • Recombinant Proteins
  • Peptides
  • Oocytes
  • Molecular Chaperones
  • Mitochondria
  • In Vitro Techniques
  • Genetic Variation
  • Female