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Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector.

Publication ,  Journal Article
Kornbluth, S; Cross, FR; Harbison, M; Hanafusa, H
Published in: Molecular and cellular biology
May 1986

The middle T antigen of polyomavirus transformed primary chicken embryo fibroblasts when expressed from a replication-competent avian retrovirus. This in vitro-constructed retrovirus, SRMT1, is a variant of Rous sarcoma virus that encodes the middle T antigen in place of v-src. Inoculation of SRMT1 into 1-week-old chickens rapidly induced hemangiomas and hemangiosarcomas. As shown with mammalian cells infected with polyomavirus, polyomavirus middle T antigen appears to be associated with p60c-src in chicken cells infected with SRMT1. When lysates of SRMT1-infected cells immunoprecipitated with either a monoclonal antibody against p60src or anti-T serum were assayed in an in vitro kinase reaction, the middle T antigen was heavily phosphorylated. To see whether an excess of p60c-src could alter the extent of phosphorylation of the middle T protein or the process of cell transformation by middle T, cells were doubly infected with SRMT1 and NY501, a virus which overexpresses p60c-src. Doubly infected chicken embryo fibroblasts transformed with the same kinetics and were morphologically indistinguishable from chicken embryo fibroblasts infected with SRMT1 alone. Phosphorylation of the middle T antigen was elevated two- to fivefold relative to cells infected only with SRMT1.

Duke Scholars

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

May 1986

Volume

6

Issue

5

Start / End Page

1545 / 1551

Related Subject Headings

  • Transfection
  • Sarcoma, Avian
  • Rats
  • Protein Kinases
  • Polyomavirus
  • Plasmids
  • Oncogene Proteins, Viral
  • Genetic Vectors
  • Fibroblasts
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
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Kornbluth, S., Cross, F. R., Harbison, M., & Hanafusa, H. (1986). Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector. Molecular and Cellular Biology, 6(5), 1545–1551. https://doi.org/10.1128/mcb.6.5.1545-1551.1986
Kornbluth, S., F. R. Cross, M. Harbison, and H. Hanafusa. “Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector.Molecular and Cellular Biology 6, no. 5 (May 1986): 1545–51. https://doi.org/10.1128/mcb.6.5.1545-1551.1986.
Kornbluth S, Cross FR, Harbison M, Hanafusa H. Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector. Molecular and cellular biology. 1986 May;6(5):1545–51.
Kornbluth, S., et al. “Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector.Molecular and Cellular Biology, vol. 6, no. 5, May 1986, pp. 1545–51. Epmc, doi:10.1128/mcb.6.5.1545-1551.1986.
Kornbluth S, Cross FR, Harbison M, Hanafusa H. Transformation of chicken embryo fibroblasts and tumor induction by the middle T antigen of polyomavirus carried in an avian retroviral vector. Molecular and cellular biology. 1986 May;6(5):1545–1551.

Published In

Molecular and cellular biology

DOI

EISSN

1098-5549

ISSN

0270-7306

Publication Date

May 1986

Volume

6

Issue

5

Start / End Page

1545 / 1551

Related Subject Headings

  • Transfection
  • Sarcoma, Avian
  • Rats
  • Protein Kinases
  • Polyomavirus
  • Plasmids
  • Oncogene Proteins, Viral
  • Genetic Vectors
  • Fibroblasts
  • Developmental Biology