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Multiple effects of N, N' dicyclohexyl carbodiimide on the beta-adrenergic receptor--adenylate cyclase system in frog erythrocytes.

Publication ,  Journal Article
Pike, LJ; Lefkowitz, RJ
Published in: J Cyclic Nucleotide Res
February 1978

Treatment of frog erythrocytes with N,N' dicyclohexylcarbodiimide (DCCD) leads to a loss of catecholamine stimulated adenylate cyclase activity without any decrease in fluoride or PGE1 stimulated cyclase. However, the concentrations of the reagent which inhibit catecholamine sensitive adenylate cyclase activity are 10 fold lower than those which inhibit specific [3H]dihydroalprenolol ([3H]DHA) beta-adrenergic receptor binding. By contrast binding of the readiolabeled beta-adrenergic agonist [3H]hydroxybenzylisoproterenol ([3H]HBI) is considerably more sensitive than antagonist binding to the effects of DCCD. The data suggest that low concentrations of the reagent may modify the effector portion of the beta-adrenergic receptor leading to functional uncoupling of the beta-receptor adenylate cyclase system. At higher concentrations of the reagent the ligand bidning site of the beta-receptor appears also to be altered.

Duke Scholars

Published In

J Cyclic Nucleotide Res

ISSN

0095-1544

Publication Date

February 1978

Volume

4

Issue

1

Start / End Page

27 / 34

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Prostaglandins E
  • Kinetics
  • Isoproterenol
  • Erythrocytes
  • Erythrocyte Membrane
  • Dicyclohexylcarbodiimide
  • Carbodiimides
  • Anura
 

Citation

APA
Chicago
ICMJE
MLA
NLM

Published In

J Cyclic Nucleotide Res

ISSN

0095-1544

Publication Date

February 1978

Volume

4

Issue

1

Start / End Page

27 / 34

Location

United States

Related Subject Headings

  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Prostaglandins E
  • Kinetics
  • Isoproterenol
  • Erythrocytes
  • Erythrocyte Membrane
  • Dicyclohexylcarbodiimide
  • Carbodiimides
  • Anura