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Identification of the cardiac beta-adrenergic receptor protein: solubilization and purification by affinity chromatography.

Publication ,  Journal Article
Lefkowitz, RJ; Haber, E; O'Hara, D
Published in: Proc Natl Acad Sci U S A
October 1972

A protein that binds catecholamines with a specificity parallel to that of their in vivo effects on cardiac contractility (isoproterenol > epinephrine or norepinephrine > dopamine > dihydroxyphenylalanine) was solubilized from a microsomal fraction of canine ventricular myocardium. The binding protein was purified 500 to 800-fold by solubilization and subsequent affinity chromatography with conjugates of norepinephrine linked to agarose beads. Purified beta-adrenergic binding protein exists in two forms, corresponding to molecular weights of 40,000 and 160,000. The purified material has a single association constant, 2.3 x 10(5) liters/mol (as compared to two association constants, 10(7) and 10(6) liters/mol, for the binding protein in particulate form) but retains the identical binding specificity for beta-adrenergic drugs and antagonists.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1972

Volume

69

Issue

10

Start / End Page

2828 / 2832

Location

United States

Related Subject Headings

  • Tritium
  • Succinates
  • Solubility
  • Receptors, Adrenergic
  • Protein Binding
  • Propylamines
  • Polysaccharides
  • Norepinephrine
  • Myocardium
  • Muscle Proteins
 

Citation

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Lefkowitz, R. J., Haber, E., & O’Hara, D. (1972). Identification of the cardiac beta-adrenergic receptor protein: solubilization and purification by affinity chromatography. Proc Natl Acad Sci U S A, 69(10), 2828–2832. https://doi.org/10.1073/pnas.69.10.2828
Lefkowitz, R. J., E. Haber, and D. O’Hara. “Identification of the cardiac beta-adrenergic receptor protein: solubilization and purification by affinity chromatography.Proc Natl Acad Sci U S A 69, no. 10 (October 1972): 2828–32. https://doi.org/10.1073/pnas.69.10.2828.
Lefkowitz RJ, Haber E, O’Hara D. Identification of the cardiac beta-adrenergic receptor protein: solubilization and purification by affinity chromatography. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2828–32.
Lefkowitz, R. J., et al. “Identification of the cardiac beta-adrenergic receptor protein: solubilization and purification by affinity chromatography.Proc Natl Acad Sci U S A, vol. 69, no. 10, Oct. 1972, pp. 2828–32. Pubmed, doi:10.1073/pnas.69.10.2828.
Lefkowitz RJ, Haber E, O’Hara D. Identification of the cardiac beta-adrenergic receptor protein: solubilization and purification by affinity chromatography. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2828–2832.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

October 1972

Volume

69

Issue

10

Start / End Page

2828 / 2832

Location

United States

Related Subject Headings

  • Tritium
  • Succinates
  • Solubility
  • Receptors, Adrenergic
  • Protein Binding
  • Propylamines
  • Polysaccharides
  • Norepinephrine
  • Myocardium
  • Muscle Proteins