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Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma.

Publication ,  Journal Article
Lefkowitz, RJ; Roth, J; Pastan, I
Published in: Science
November 6, 1970

Biologically active iodine-125-labeled adrenocorticotropic hormone (ACTH) binds specifically to ACTH receptors extracted from adrenals. Unlabeled ACTH at 1 picogram per milliliter significantly displaces labeled ACTH from these receptors. This system, which appears to be applicable to all polypeptide hormones, provides a rapid and sensitive method for measurements of biologically active ACTH in dilute whole plasma.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

November 6, 1970

Volume

170

Issue

3958

Start / End Page

633 / 635

Location

United States

Related Subject Headings

  • Swine
  • Receptors, Drug
  • Radioimmunoassay
  • Peptides
  • Methods
  • Iodine Isotopes
  • Hypopituitarism
  • Humans
  • Hormones
  • General Science & Technology
 

Citation

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Lefkowitz, R. J., Roth, J., & Pastan, I. (1970). Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma. Science, 170(3958), 633–635. https://doi.org/10.1126/science.170.3958.633
Lefkowitz, R. J., J. Roth, and I. Pastan. “Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma.Science 170, no. 3958 (November 6, 1970): 633–35. https://doi.org/10.1126/science.170.3958.633.
Lefkowitz, R. J., et al. “Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma.Science, vol. 170, no. 3958, Nov. 1970, pp. 633–35. Pubmed, doi:10.1126/science.170.3958.633.
Lefkowitz RJ, Roth J, Pastan I. Radioreceptor assay of adrenocorticotropic hormone: new approach to assay of polypeptide hormones in plasma. Science. 1970 Nov 6;170(3958):633–635.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

November 6, 1970

Volume

170

Issue

3958

Start / End Page

633 / 635

Location

United States

Related Subject Headings

  • Swine
  • Receptors, Drug
  • Radioimmunoassay
  • Peptides
  • Methods
  • Iodine Isotopes
  • Hypopituitarism
  • Humans
  • Hormones
  • General Science & Technology