ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase.
Publication
, Journal Article
Lefkowitz, RJ; Roth, J; Pricer, W; Pastan, I
Published in: Proc Natl Acad Sci U S A
March 1970
Pure monoiodo ACTH-(125)I was prepared that was biologically active and free of unlabeled ACTH. Extracts of adrenal cortex that contained ACTH-sensitive adenyl cyclase, bound ACTH-(125)I; extracts that lacked the ACTH-sensitive cyclase did not bind ACTH-(125)I. Unlabeled ACTH inhibited the binding of ACTH-(125)I. Five ACTH derivatives which varied widely in biological activity were tested. All inhibited the binding of ACTH-(125)I in direct proportion to their biological activity. Albumin, insulin, and four unrelated iodinated hormones were inert. The addition of excess hormone or acetic acid produced rapid dissociation of bound ACTH-(125)I. This study demonstrates directly the binding of ACTH to its biologically significant site.
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Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
March 1970
Volume
65
Issue
3
Start / End Page
745 / 752
Location
United States
Related Subject Headings
- Neoplasms, Experimental
- Mice
- Iodine Radioisotopes
- Chemoreceptor Cells
- Biological Assay
- Binding Sites
- Animals
- Adrenocorticotropic Hormone
- Adrenal Glands
- Adrenal Gland Neoplasms
Citation
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ICMJE
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Lefkowitz, R. J., Roth, J., Pricer, W., & Pastan, I. (1970). ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase. Proc Natl Acad Sci U S A, 65(3), 745–752. https://doi.org/10.1073/pnas.65.3.745
Lefkowitz, R. J., J. Roth, W. Pricer, and I. Pastan. “ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase.” Proc Natl Acad Sci U S A 65, no. 3 (March 1970): 745–52. https://doi.org/10.1073/pnas.65.3.745.
Lefkowitz RJ, Roth J, Pricer W, Pastan I. ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase. Proc Natl Acad Sci U S A. 1970 Mar;65(3):745–52.
Lefkowitz, R. J., et al. “ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase.” Proc Natl Acad Sci U S A, vol. 65, no. 3, Mar. 1970, pp. 745–52. Pubmed, doi:10.1073/pnas.65.3.745.
Lefkowitz RJ, Roth J, Pricer W, Pastan I. ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase. Proc Natl Acad Sci U S A. 1970 Mar;65(3):745–752.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
March 1970
Volume
65
Issue
3
Start / End Page
745 / 752
Location
United States
Related Subject Headings
- Neoplasms, Experimental
- Mice
- Iodine Radioisotopes
- Chemoreceptor Cells
- Biological Assay
- Binding Sites
- Animals
- Adrenocorticotropic Hormone
- Adrenal Glands
- Adrenal Gland Neoplasms