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Effects of quercetin on magnesium-dependent adenosine triphosphatase and the metabolism of human polymorphonuclear leukocytes.

Publication ,  Journal Article
Long, GD; DeChatelet, LR; O'Flaherty, JT; McCall, CE; Bass, DA; Shirley, PS; Parce, JW
Published in: Blood
March 1981

The bioflavinoid quercetin was found to exert at least three separate effects on human polymorphonuclear leukocytes. (1) Concentrations of approximately 100 microM inhibited the membrane-associated magnesium adenosine triphosphatase by 60%-80% in either broken cell preparations or intact cells. Lineweaver-Burk plots showed the inhibition to be uncompetitive in nature. (2) Similar concentrations of quercetin inhibited respiratory burst activity of the cells as measured by oxygen consumption, glucose oxidation, or iodination of protein. All inhibitions were dose-dependent and were observed with either opsonized zymosan or phorbol myristate acetate as stimulus. (3) Quercetin likewise inhibited the transport of the nonmetabolizable hexose, 3H-2-deoxyglucose. These observations are most consistent with the hypothesis that quercetin exerts a generalized effect at the level of the cell membrane of the neutrophil.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

March 1981

Volume

57

Issue

3

Start / End Page

561 / 566

Location

United States

Related Subject Headings

  • Quercetin
  • Protein Binding
  • Oxygen Consumption
  • Neutrophils
  • Magnesium
  • Iodine
  • Immunology
  • Humans
  • Glucose
  • Flavonoids
 

Citation

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ICMJE
MLA
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Long, G. D., DeChatelet, L. R., O’Flaherty, J. T., McCall, C. E., Bass, D. A., Shirley, P. S., & Parce, J. W. (1981). Effects of quercetin on magnesium-dependent adenosine triphosphatase and the metabolism of human polymorphonuclear leukocytes. Blood, 57(3), 561–566.
Long, G. D., L. R. DeChatelet, J. T. O’Flaherty, C. E. McCall, D. A. Bass, P. S. Shirley, and J. W. Parce. “Effects of quercetin on magnesium-dependent adenosine triphosphatase and the metabolism of human polymorphonuclear leukocytes.Blood 57, no. 3 (March 1981): 561–66.
Long GD, DeChatelet LR, O’Flaherty JT, McCall CE, Bass DA, Shirley PS, et al. Effects of quercetin on magnesium-dependent adenosine triphosphatase and the metabolism of human polymorphonuclear leukocytes. Blood. 1981 Mar;57(3):561–6.
Long GD, DeChatelet LR, O’Flaherty JT, McCall CE, Bass DA, Shirley PS, Parce JW. Effects of quercetin on magnesium-dependent adenosine triphosphatase and the metabolism of human polymorphonuclear leukocytes. Blood. 1981 Mar;57(3):561–566.

Published In

Blood

ISSN

0006-4971

Publication Date

March 1981

Volume

57

Issue

3

Start / End Page

561 / 566

Location

United States

Related Subject Headings

  • Quercetin
  • Protein Binding
  • Oxygen Consumption
  • Neutrophils
  • Magnesium
  • Iodine
  • Immunology
  • Humans
  • Glucose
  • Flavonoids