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Genome-wide localization of replication factors.

Publication ,  Journal Article
Lubelsky, Y; MacAlpine, HK; MacAlpine, DM
Published in: Methods
June 2012

Chromatin Immunoprecipitation (ChIP) is a powerful tool for the identification and characterization of protein-DNA interactions in vivo. ChIP has been utilized to study diverse nuclear processes such as transcription regulation, chromatin modification, DNA recombination and DNA replication at specific loci and, more recently, across the entire genome. Advances in genomic approaches, and whole genome sequencing in particular, have made it possible and affordable to comprehensively identify specific protein binding sites throughout the genomes of higher eukaryotes. The dynamic nature of the DNA replication program and the transient occupancy of many replication factors throughout the cell cycle present additional challenges that may not pertain to the mapping of site specific transcription factors. Here we discuss the specific considerations that need to be addressed in the application of ChIP to the genome-wide location analysis of protein factors involved in DNA replication.

Duke Scholars

Published In

Methods

DOI

EISSN

1095-9130

Publication Date

June 2012

Volume

57

Issue

2

Start / End Page

187 / 195

Location

United States

Related Subject Headings

  • Tissue Fixation
  • Replication Origin
  • Protein Binding
  • Polymerase Chain Reaction
  • Oligonucleotide Array Sequence Analysis
  • High-Throughput Nucleotide Sequencing
  • Gene Library
  • Formaldehyde
  • Fixatives
  • Drosophila Proteins
 

Citation

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Lubelsky, Y., MacAlpine, H. K., & MacAlpine, D. M. (2012). Genome-wide localization of replication factors. Methods, 57(2), 187–195. https://doi.org/10.1016/j.ymeth.2012.03.022
Lubelsky, Yoav, Heather K. MacAlpine, and David M. MacAlpine. “Genome-wide localization of replication factors.Methods 57, no. 2 (June 2012): 187–95. https://doi.org/10.1016/j.ymeth.2012.03.022.
Lubelsky Y, MacAlpine HK, MacAlpine DM. Genome-wide localization of replication factors. Methods. 2012 Jun;57(2):187–95.
Lubelsky, Yoav, et al. “Genome-wide localization of replication factors.Methods, vol. 57, no. 2, June 2012, pp. 187–95. Pubmed, doi:10.1016/j.ymeth.2012.03.022.
Lubelsky Y, MacAlpine HK, MacAlpine DM. Genome-wide localization of replication factors. Methods. 2012 Jun;57(2):187–195.
Journal cover image

Published In

Methods

DOI

EISSN

1095-9130

Publication Date

June 2012

Volume

57

Issue

2

Start / End Page

187 / 195

Location

United States

Related Subject Headings

  • Tissue Fixation
  • Replication Origin
  • Protein Binding
  • Polymerase Chain Reaction
  • Oligonucleotide Array Sequence Analysis
  • High-Throughput Nucleotide Sequencing
  • Gene Library
  • Formaldehyde
  • Fixatives
  • Drosophila Proteins