Skip to main content

Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea.

Publication ,  Journal Article
Merson, MH; Sack, RB; Kibriya, AK; Al-Mahmood, A; Adamed, QS; Huq, I
Published in: J Clin Microbiol
April 1979

Diagnosis of enterotoxigenic Escherichia coli diarrhea was made in 109 adult males with an acute dehydrating cholera-like syndrome in Dacca, Bangladesh, by testing 10 colonies isolated from admission stool specimens for production of heat-labile and heat-stable toxins. Toxin testing of one colony yielded a diagnosis in 92% of the cases, testing of two colonies yielded a diagnosis in 95% of the cases, testing of a pool of 5 colonies yielded a diagnosis in 95% of the cases, and testing of a pool of 10 colonies yielded a diagnosis in 96% of the cases. From stool cultures obtained on subsequent days, toxin testing of individual colonies and pools revealed diminished efficacy of pooling with decreasing numbers of enterotoxin-positive isolates in the pool. To detect the presence of enterotoxigenic E. coli in stools, toxin testing of 5 individual isolates and a pool of 10 colonies was found to be almost as effective as the testing of 10 individual isolates.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1979

Volume

9

Issue

4

Start / End Page

493 / 497

Location

United States

Related Subject Headings

  • Tetracycline
  • Microbiology
  • Male
  • Humans
  • Feces
  • Escherichia coli Infections
  • Escherichia coli
  • Enterotoxins
  • Diarrhea
  • Diagnosis, Differential
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Merson, M. H., Sack, R. B., Kibriya, A. K., Al-Mahmood, A., Adamed, Q. S., & Huq, I. (1979). Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea. J Clin Microbiol, 9(4), 493–497. https://doi.org/10.1128/jcm.9.4.493-497.1979
Merson, M. H., R. B. Sack, A. K. Kibriya, A. Al-Mahmood, Q. S. Adamed, and I. Huq. “Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea.J Clin Microbiol 9, no. 4 (April 1979): 493–97. https://doi.org/10.1128/jcm.9.4.493-497.1979.
Merson MH, Sack RB, Kibriya AK, Al-Mahmood A, Adamed QS, Huq I. Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea. J Clin Microbiol. 1979 Apr;9(4):493–7.
Merson, M. H., et al. “Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea.J Clin Microbiol, vol. 9, no. 4, Apr. 1979, pp. 493–97. Pubmed, doi:10.1128/jcm.9.4.493-497.1979.
Merson MH, Sack RB, Kibriya AK, Al-Mahmood A, Adamed QS, Huq I. Use of colony pools for diagnosis of enterotoxigenic Escherichia coli diarrhea. J Clin Microbiol. 1979 Apr;9(4):493–497.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1979

Volume

9

Issue

4

Start / End Page

493 / 497

Location

United States

Related Subject Headings

  • Tetracycline
  • Microbiology
  • Male
  • Humans
  • Feces
  • Escherichia coli Infections
  • Escherichia coli
  • Enterotoxins
  • Diarrhea
  • Diagnosis, Differential