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Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli.

Publication ,  Journal Article
Morris, GK; Merson, MH; Sack, DA; Wells, JG; Martin, WT; Dewitt, WE; Feeley, JC; Sack, RB; Bessudo, DM
Published in: J Clin Microbiol
May 1976

A laboratory investigation was conducted on cultures collected from travelers before, during, and after a trip to Mexico to characterize the etiology of traveler's diarrhea. Four laboratory methods for detecting enterotoxigenicity of Escherichia coli were evaluated: the infant mouse assay, the Chinese hamster ovary (CHO) cell assay, the Y1 adrenal cell assay, and the rabbit ileal loop. Although a number of common enteric pathogens were identified as a cause of traveler's diarrhea, including six serotypes of Salmonella, two serotypes of Shigella, Vibrio parahaemolyticus, Giardia lamblia, and Entamoeba histolytica, enterotoxigenic Escherichia coli was most commonly isolated. Strains were identified that produced only heat-labile enterotoxin (LT), only heat-stable enterotoxin (ST), or both LT and ST. The infant mouse assay yielded results falling into two distinct groups, providing a clear separation of positive and negative cultures. The CHO assay also formed two groups, with positive cultures producing 11% or more of the elongated cells. There was good agreement between the CHO and the Y1 adrenal cell assays for detection of LT. The adrenal cell system for detection of LT was more suitable than the CHO assay for processing large numbers of specimens because of the miniculture modification of this method utilized in this study. The infant mouse method was a simple and reliable method for detecting ST.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

May 1976

Volume

3

Issue

5

Start / End Page

486 / 495

Location

United States

Related Subject Headings

  • Vibrio parahaemolyticus
  • Travel
  • Rabbits
  • Microbiology
  • Mice
  • Mexico
  • Humans
  • Giardia
  • Feces
  • Evaluation Studies as Topic
 

Citation

APA
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ICMJE
MLA
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Morris, G. K., Merson, M. H., Sack, D. A., Wells, J. G., Martin, W. T., Dewitt, W. E., … Bessudo, D. M. (1976). Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli. J Clin Microbiol, 3(5), 486–495. https://doi.org/10.1128/jcm.3.5.486-495.1976
Morris, G. K., M. H. Merson, D. A. Sack, J. G. Wells, W. T. Martin, W. E. Dewitt, J. C. Feeley, R. B. Sack, and D. M. Bessudo. “Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli.J Clin Microbiol 3, no. 5 (May 1976): 486–95. https://doi.org/10.1128/jcm.3.5.486-495.1976.
Morris GK, Merson MH, Sack DA, Wells JG, Martin WT, Dewitt WE, et al. Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli. J Clin Microbiol. 1976 May;3(5):486–95.
Morris, G. K., et al. “Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli.J Clin Microbiol, vol. 3, no. 5, May 1976, pp. 486–95. Pubmed, doi:10.1128/jcm.3.5.486-495.1976.
Morris GK, Merson MH, Sack DA, Wells JG, Martin WT, Dewitt WE, Feeley JC, Sack RB, Bessudo DM. Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli. J Clin Microbiol. 1976 May;3(5):486–495.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

May 1976

Volume

3

Issue

5

Start / End Page

486 / 495

Location

United States

Related Subject Headings

  • Vibrio parahaemolyticus
  • Travel
  • Rabbits
  • Microbiology
  • Mice
  • Mexico
  • Humans
  • Giardia
  • Feces
  • Evaluation Studies as Topic