
PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.
Publication
, Journal Article
van Oers, JMM; Roa, S; Werling, U; Liu, Y; Genschel, J; Hou, H; Sellers, RS; Modrich, P; Scharff, MD; Edelmann, W
Published in: Proc Natl Acad Sci U S A
July 27, 2010
The DNA mismatch repair protein PMS2 was recently found to encode a novel endonuclease activity. To determine the biological functions of this activity in mammals, we generated endonuclease-deficient Pms2E702K knock-in mice. Pms2EK/EK mice displayed increased genomic mutation rates and a strong cancer predisposition. In addition, class switch recombination, but not somatic hypermutation, was impaired in Pms2EK/EK B cells, indicating a specific role in Ig diversity. In contrast to Pms2-/- mice, Pms2EK/EK male mice were fertile, indicating that this activity is dispensable in spermatogenesis. Therefore, the PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance and tumor suppression.
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Published In
Proc Natl Acad Sci U S A
DOI
EISSN
1091-6490
Publication Date
July 27, 2010
Volume
107
Issue
30
Start / End Page
13384 / 13389
Location
United States
Related Subject Headings
- Reverse Transcriptase Polymerase Chain Reaction
- Mutation
- Mismatch Repair Endonuclease PMS2
- Mice, Knockout
- Mice
- Male
- Lymphoma
- Immunoglobulin G
- Immunoglobulin Class Switching
- Humans
Citation
APA
Chicago
ICMJE
MLA
NLM
van Oers, J. M. M., Roa, S., Werling, U., Liu, Y., Genschel, J., Hou, H., … Edelmann, W. (2010). PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A, 107(30), 13384–13389. https://doi.org/10.1073/pnas.1008589107
Oers, Johanna M. M. van, Sergio Roa, Uwe Werling, Yiyong Liu, Jochen Genschel, Harry Hou, Rani S. Sellers, Paul Modrich, Matthew D. Scharff, and Winfried Edelmann. “PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.” Proc Natl Acad Sci U S A 107, no. 30 (July 27, 2010): 13384–89. https://doi.org/10.1073/pnas.1008589107.
van Oers JMM, Roa S, Werling U, Liu Y, Genschel J, Hou H, et al. PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13384–9.
van Oers, Johanna M. M., et al. “PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.” Proc Natl Acad Sci U S A, vol. 107, no. 30, July 2010, pp. 13384–89. Pubmed, doi:10.1073/pnas.1008589107.
van Oers JMM, Roa S, Werling U, Liu Y, Genschel J, Hou H, Sellers RS, Modrich P, Scharff MD, Edelmann W. PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13384–13389.

Published In
Proc Natl Acad Sci U S A
DOI
EISSN
1091-6490
Publication Date
July 27, 2010
Volume
107
Issue
30
Start / End Page
13384 / 13389
Location
United States
Related Subject Headings
- Reverse Transcriptase Polymerase Chain Reaction
- Mutation
- Mismatch Repair Endonuclease PMS2
- Mice, Knockout
- Mice
- Male
- Lymphoma
- Immunoglobulin G
- Immunoglobulin Class Switching
- Humans