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PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.

Publication ,  Journal Article
van Oers, JMM; Roa, S; Werling, U; Liu, Y; Genschel, J; Hou, H; Sellers, RS; Modrich, P; Scharff, MD; Edelmann, W
Published in: Proc Natl Acad Sci U S A
July 27, 2010

The DNA mismatch repair protein PMS2 was recently found to encode a novel endonuclease activity. To determine the biological functions of this activity in mammals, we generated endonuclease-deficient Pms2E702K knock-in mice. Pms2EK/EK mice displayed increased genomic mutation rates and a strong cancer predisposition. In addition, class switch recombination, but not somatic hypermutation, was impaired in Pms2EK/EK B cells, indicating a specific role in Ig diversity. In contrast to Pms2-/- mice, Pms2EK/EK male mice were fertile, indicating that this activity is dispensable in spermatogenesis. Therefore, the PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance and tumor suppression.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

July 27, 2010

Volume

107

Issue

30

Start / End Page

13384 / 13389

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Mutation
  • Mismatch Repair Endonuclease PMS2
  • Mice, Knockout
  • Mice
  • Male
  • Lymphoma
  • Immunoglobulin G
  • Immunoglobulin Class Switching
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
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van Oers, J. M. M., Roa, S., Werling, U., Liu, Y., Genschel, J., Hou, H., … Edelmann, W. (2010). PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A, 107(30), 13384–13389. https://doi.org/10.1073/pnas.1008589107
Oers, Johanna M. M. van, Sergio Roa, Uwe Werling, Yiyong Liu, Jochen Genschel, Harry Hou, Rani S. Sellers, Paul Modrich, Matthew D. Scharff, and Winfried Edelmann. “PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.Proc Natl Acad Sci U S A 107, no. 30 (July 27, 2010): 13384–89. https://doi.org/10.1073/pnas.1008589107.
van Oers JMM, Roa S, Werling U, Liu Y, Genschel J, Hou H, et al. PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13384–9.
van Oers, Johanna M. M., et al. “PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.Proc Natl Acad Sci U S A, vol. 107, no. 30, July 2010, pp. 13384–89. Pubmed, doi:10.1073/pnas.1008589107.
van Oers JMM, Roa S, Werling U, Liu Y, Genschel J, Hou H, Sellers RS, Modrich P, Scharff MD, Edelmann W. PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13384–13389.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

July 27, 2010

Volume

107

Issue

30

Start / End Page

13384 / 13389

Location

United States

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Mutation
  • Mismatch Repair Endonuclease PMS2
  • Mice, Knockout
  • Mice
  • Male
  • Lymphoma
  • Immunoglobulin G
  • Immunoglobulin Class Switching
  • Humans