
Mapping individual cosmid DNAs by direct AFM imaging.
Publication
, Journal Article
Allison, DP; Kerper, PS; Doktycz, MJ; Thundat, T; Modrich, P; Larimer, FW; Johnson, DK; Hoyt, PR; Mucenski, ML; Warmack, RJ
Published in: Genomics
May 1, 1997
Individual cosmid clones have been restriction mapped by directly imaging, with the atomic force microscope (AFM), a mutant EcoRI endonuclease site-specifically bound to DNA. Images and data are presented that locate six restriction sites, predicted from gel electrophoresis, on a 35-kb cosmid isolated from mouse chromosome 7. Measured distances between endonuclease molecules bound to lambda DNA, when compared to known values, demonstrate the accuracy of AFM mapping to better than 1%. These results may be extended to identify other important site-specific protein-DNA interactions, such as transcription factor and mismatch repair enzyme binding, difficult to resolve by current techniques.
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Published In
Genomics
DOI
ISSN
0888-7543
Publication Date
May 1, 1997
Volume
41
Issue
3
Start / End Page
379 / 384
Location
United States
Related Subject Headings
- Protein Binding
- Microscopy, Atomic Force
- Mice
- Image Processing, Computer-Assisted
- Genetics & Heredity
- DNA
- Cosmids
- Cloning, Molecular
- Chromosome Mapping
- Binding Sites
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Chicago
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Allison, D. P., Kerper, P. S., Doktycz, M. J., Thundat, T., Modrich, P., Larimer, F. W., … Warmack, R. J. (1997). Mapping individual cosmid DNAs by direct AFM imaging. Genomics, 41(3), 379–384. https://doi.org/10.1006/geno.1997.4686
Allison, D. P., P. S. Kerper, M. J. Doktycz, T. Thundat, P. Modrich, F. W. Larimer, D. K. Johnson, P. R. Hoyt, M. L. Mucenski, and R. J. Warmack. “Mapping individual cosmid DNAs by direct AFM imaging.” Genomics 41, no. 3 (May 1, 1997): 379–84. https://doi.org/10.1006/geno.1997.4686.
Allison DP, Kerper PS, Doktycz MJ, Thundat T, Modrich P, Larimer FW, et al. Mapping individual cosmid DNAs by direct AFM imaging. Genomics. 1997 May 1;41(3):379–84.
Allison, D. P., et al. “Mapping individual cosmid DNAs by direct AFM imaging.” Genomics, vol. 41, no. 3, May 1997, pp. 379–84. Pubmed, doi:10.1006/geno.1997.4686.
Allison DP, Kerper PS, Doktycz MJ, Thundat T, Modrich P, Larimer FW, Johnson DK, Hoyt PR, Mucenski ML, Warmack RJ. Mapping individual cosmid DNAs by direct AFM imaging. Genomics. 1997 May 1;41(3):379–384.

Published In
Genomics
DOI
ISSN
0888-7543
Publication Date
May 1, 1997
Volume
41
Issue
3
Start / End Page
379 / 384
Location
United States
Related Subject Headings
- Protein Binding
- Microscopy, Atomic Force
- Mice
- Image Processing, Computer-Assisted
- Genetics & Heredity
- DNA
- Cosmids
- Cloning, Molecular
- Chromosome Mapping
- Binding Sites