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Partial NH2- and cooh-terminal sequence analyses of Eco RI DNA restriction and modification enzymes.

Publication ,  Journal Article
Rubin, RA; Modrich, P; Vanaman, TC
Published in: J Biol Chem
March 10, 1981

NH2- and COOH-terminal amino acid sequences of the Eco RI restriction and modification enzymes have been determined. The results allow localization of the coding regions within the DNA segment which controls activity of both enzymes. Processing of the endonuclease is limited to removal of NH2-terminal formylmethionine whereas, in the case of the methylase, formylMet-Ala is removed.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

March 10, 1981

Volume

256

Issue

5

Start / End Page

2140 / 2142

Location

United States

Related Subject Headings

  • Methyltransferases
  • Genes
  • Escherichia coli
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • DNA (Cytosine-5-)-Methyltransferases
  • Biochemistry & Molecular Biology
  • Amino Acid Sequence
  • 34 Chemical sciences
  • 32 Biomedical and clinical sciences
 

Citation

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Rubin, R. A., Modrich, P., & Vanaman, T. C. (1981). Partial NH2- and cooh-terminal sequence analyses of Eco RI DNA restriction and modification enzymes. J Biol Chem, 256(5), 2140–2142.
Rubin, R. A., P. Modrich, and T. C. Vanaman. “Partial NH2- and cooh-terminal sequence analyses of Eco RI DNA restriction and modification enzymes.J Biol Chem 256, no. 5 (March 10, 1981): 2140–42.
Rubin RA, Modrich P, Vanaman TC. Partial NH2- and cooh-terminal sequence analyses of Eco RI DNA restriction and modification enzymes. J Biol Chem. 1981 Mar 10;256(5):2140–2.
Rubin, R. A., et al. “Partial NH2- and cooh-terminal sequence analyses of Eco RI DNA restriction and modification enzymes.J Biol Chem, vol. 256, no. 5, Mar. 1981, pp. 2140–42.
Rubin RA, Modrich P, Vanaman TC. Partial NH2- and cooh-terminal sequence analyses of Eco RI DNA restriction and modification enzymes. J Biol Chem. 1981 Mar 10;256(5):2140–2142.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

March 10, 1981

Volume

256

Issue

5

Start / End Page

2140 / 2142

Location

United States

Related Subject Headings

  • Methyltransferases
  • Genes
  • Escherichia coli
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • DNA (Cytosine-5-)-Methyltransferases
  • Biochemistry & Molecular Biology
  • Amino Acid Sequence
  • 34 Chemical sciences
  • 32 Biomedical and clinical sciences