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Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease.

Publication ,  Journal Article
Geier, GE; Modrich, P
Published in: J Biol Chem
February 25, 1979

The recognition sequence for the dam methylase of Escherichia coli K12 has been determined directly by use of in vivo methylated ColE1 DNA or DNA methylated in vitro with purified enzyme. The methylase recognizes the symmetric tetranucleotide d(pG-A-T-C) and introduces two methyl groups per site in duplex DNA with the product of methylation being 6-methylaminopurine. This work has also demonstrated that Dpn I restriction endonuclease cleaves on the 3' side of the modified adenine within the methylated sequence to yield DNA fragments possessing fully base-paired termini. All sequences in ColE1 DNA methylated by the dam enzyme are subject to double strand cleavage by Dpn I endonuclease. Therefore, this restriction enzyme can be employed for mapping the location of sequences possessing the dam modification.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

February 25, 1979

Volume

254

Issue

4

Start / End Page

1408 / 1413

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Methyltransferases
  • Escherichia coli
  • Endonucleases
  • Deoxyribonucleases
  • DNA, Circular
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • DNA (Cytosine-5-)-Methyltransferases
  • Biochemistry & Molecular Biology
 

Citation

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Geier, G. E., and P. Modrich. “Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease.J Biol Chem 254, no. 4 (February 25, 1979): 1408–13.
Geier, G. E., and P. Modrich. “Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease.J Biol Chem, vol. 254, no. 4, Feb. 1979, pp. 1408–13.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

February 25, 1979

Volume

254

Issue

4

Start / End Page

1408 / 1413

Location

United States

Related Subject Headings

  • Substrate Specificity
  • Methyltransferases
  • Escherichia coli
  • Endonucleases
  • Deoxyribonucleases
  • DNA, Circular
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • DNA (Cytosine-5-)-Methyltransferases
  • Biochemistry & Molecular Biology