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Substrate dependence of the mechanism of EcoRI endonuclease.

Publication ,  Journal Article
Rubin, RA; Modrich, P
Published in: Nucleic Acids Res
August 1978

The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double strand cleavage of SV40 DNA occurs without dissociation of the enzyme in only 75% of the catalytic events. This mechanistic difference presumably reflects sequence differences about the EcoRI sites of these DNA's.

Duke Scholars

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

August 1978

Volume

5

Issue

8

Start / End Page

2991 / 2997

Location

England

Related Subject Headings

  • Substrate Specificity
  • Simian virus 40
  • Kinetics
  • Escherichia coli
  • Developmental Biology
  • DNA, Viral
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • Base Sequence
  • Bacteriocin Plasmids
 

Citation

APA
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ICMJE
MLA
NLM
Rubin, R. A., & Modrich, P. (1978). Substrate dependence of the mechanism of EcoRI endonuclease. Nucleic Acids Res, 5(8), 2991–2997. https://doi.org/10.1093/nar/5.8.2991
Rubin, R. A., and P. Modrich. “Substrate dependence of the mechanism of EcoRI endonuclease.Nucleic Acids Res 5, no. 8 (August 1978): 2991–97. https://doi.org/10.1093/nar/5.8.2991.
Rubin RA, Modrich P. Substrate dependence of the mechanism of EcoRI endonuclease. Nucleic Acids Res. 1978 Aug;5(8):2991–7.
Rubin, R. A., and P. Modrich. “Substrate dependence of the mechanism of EcoRI endonuclease.Nucleic Acids Res, vol. 5, no. 8, Aug. 1978, pp. 2991–97. Pubmed, doi:10.1093/nar/5.8.2991.
Rubin RA, Modrich P. Substrate dependence of the mechanism of EcoRI endonuclease. Nucleic Acids Res. 1978 Aug;5(8):2991–2997.
Journal cover image

Published In

Nucleic Acids Res

DOI

ISSN

0305-1048

Publication Date

August 1978

Volume

5

Issue

8

Start / End Page

2991 / 2997

Location

England

Related Subject Headings

  • Substrate Specificity
  • Simian virus 40
  • Kinetics
  • Escherichia coli
  • Developmental Biology
  • DNA, Viral
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • Base Sequence
  • Bacteriocin Plasmids