
Substrate dependence of the mechanism of EcoRI endonuclease.
Publication
, Journal Article
Rubin, RA; Modrich, P
Published in: Nucleic Acids Res
August 1978
The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double strand cleavage of SV40 DNA occurs without dissociation of the enzyme in only 75% of the catalytic events. This mechanistic difference presumably reflects sequence differences about the EcoRI sites of these DNA's.
Duke Scholars
Published In
Nucleic Acids Res
DOI
ISSN
0305-1048
Publication Date
August 1978
Volume
5
Issue
8
Start / End Page
2991 / 2997
Location
England
Related Subject Headings
- Substrate Specificity
- Simian virus 40
- Kinetics
- Escherichia coli
- Developmental Biology
- DNA, Viral
- DNA, Bacterial
- DNA Restriction Enzymes
- Base Sequence
- Bacteriocin Plasmids
Citation
APA
Chicago
ICMJE
MLA
NLM
Rubin, R. A., & Modrich, P. (1978). Substrate dependence of the mechanism of EcoRI endonuclease. Nucleic Acids Res, 5(8), 2991–2997. https://doi.org/10.1093/nar/5.8.2991
Rubin, R. A., and P. Modrich. “Substrate dependence of the mechanism of EcoRI endonuclease.” Nucleic Acids Res 5, no. 8 (August 1978): 2991–97. https://doi.org/10.1093/nar/5.8.2991.
Rubin RA, Modrich P. Substrate dependence of the mechanism of EcoRI endonuclease. Nucleic Acids Res. 1978 Aug;5(8):2991–7.
Rubin, R. A., and P. Modrich. “Substrate dependence of the mechanism of EcoRI endonuclease.” Nucleic Acids Res, vol. 5, no. 8, Aug. 1978, pp. 2991–97. Pubmed, doi:10.1093/nar/5.8.2991.
Rubin RA, Modrich P. Substrate dependence of the mechanism of EcoRI endonuclease. Nucleic Acids Res. 1978 Aug;5(8):2991–2997.

Published In
Nucleic Acids Res
DOI
ISSN
0305-1048
Publication Date
August 1978
Volume
5
Issue
8
Start / End Page
2991 / 2997
Location
England
Related Subject Headings
- Substrate Specificity
- Simian virus 40
- Kinetics
- Escherichia coli
- Developmental Biology
- DNA, Viral
- DNA, Bacterial
- DNA Restriction Enzymes
- Base Sequence
- Bacteriocin Plasmids