Modification of Escherichia coli DNA ligase by cleavage with trypsin.
Publication
, Journal Article
Panasenko, SM; Modrich, P; Lehman, IR
Published in: J Biol Chem
June 10, 1976
Limited treatment of Escherichia coli DNA ligase with trypsin results in rapid loss of DNA joining activity. However, the ability to react with DPN to form the covalent enzyme-AMP intermediate is unaffected. The cleaved enzyme is also unable to catalyze the formation of DNA-adenylate, the second covalent intermediate in the ligase-catalyzed reaction. These findings demonstrate that portions of the DNA ligase molecule that are required for phosphodiester bond formation are not required for at least one of the partial reactions catalyzed by this enzyme.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
June 10, 1976
Volume
251
Issue
11
Start / End Page
3432 / 3435
Location
United States
Related Subject Headings
- Trypsin
- Polynucleotide Ligases
- Peptide Fragments
- Nicotinamide Mononucleotide
- NAD
- Escherichia coli
- Biochemistry & Molecular Biology
- 34 Chemical sciences
- 32 Biomedical and clinical sciences
- 31 Biological sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Panasenko, S. M., Modrich, P., & Lehman, I. R. (1976). Modification of Escherichia coli DNA ligase by cleavage with trypsin. J Biol Chem, 251(11), 3432–3435.
Panasenko, S. M., P. Modrich, and I. R. Lehman. “Modification of Escherichia coli DNA ligase by cleavage with trypsin.” J Biol Chem 251, no. 11 (June 10, 1976): 3432–35.
Panasenko SM, Modrich P, Lehman IR. Modification of Escherichia coli DNA ligase by cleavage with trypsin. J Biol Chem. 1976 Jun 10;251(11):3432–5.
Panasenko, S. M., et al. “Modification of Escherichia coli DNA ligase by cleavage with trypsin.” J Biol Chem, vol. 251, no. 11, June 1976, pp. 3432–35.
Panasenko SM, Modrich P, Lehman IR. Modification of Escherichia coli DNA ligase by cleavage with trypsin. J Biol Chem. 1976 Jun 10;251(11):3432–3435.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
June 10, 1976
Volume
251
Issue
11
Start / End Page
3432 / 3435
Location
United States
Related Subject Headings
- Trypsin
- Polynucleotide Ligases
- Peptide Fragments
- Nicotinamide Mononucleotide
- NAD
- Escherichia coli
- Biochemistry & Molecular Biology
- 34 Chemical sciences
- 32 Biomedical and clinical sciences
- 31 Biological sciences