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Double-strand breaks associated with repetitive DNA can reshape the genome.

Publication ,  Journal Article
Argueso, JL; Westmoreland, J; Mieczkowski, PA; Gawel, M; Petes, TD; Resnick, MA
Published in: Proc Natl Acad Sci U S A
August 19, 2008

Ionizing radiation is an established source of chromosome aberrations (CAs). Although double-strand breaks (DSBs) are implicated in radiation-induced and other CAs, the underlying mechanisms are poorly understood. Here, we show that, although the vast majority of randomly induced DSBs in G(2) diploid yeast cells are repaired efficiently through homologous recombination (HR) between sister chromatids or homologous chromosomes, approximately 2% of all DSBs give rise to CAs. Complete molecular analysis of the genome revealed that nearly all of the CAs resulted from HR between nonallelic repetitive elements, primarily Ty retrotransposons. Nonhomologous end-joining (NHEJ) accounted for few, if any, of the CAs. We conclude that only those DSBs that fall at the 3-5% of the genome composed of repetitive DNA elements are efficient at generating rearrangements with dispersed small repeats across the genome, whereas DSBs in unique sequences are confined to recombinational repair between the large regions of homology contained in sister chromatids or homologous chromosomes. Because repeat-associated DSBs can efficiently lead to CAs and reshape the genome, they could be a rich source of evolutionary change.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

August 19, 2008

Volume

105

Issue

33

Start / End Page

11845 / 11850

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Oligonucleotide Array Sequence Analysis
  • Models, Genetic
  • Genome, Fungal
  • DNA Repair
  • DNA Breaks, Double-Stranded
  • DNA
  • Chromosomes, Fungal
 

Citation

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Argueso, J. L., Westmoreland, J., Mieczkowski, P. A., Gawel, M., Petes, T. D., & Resnick, M. A. (2008). Double-strand breaks associated with repetitive DNA can reshape the genome. Proc Natl Acad Sci U S A, 105(33), 11845–11850. https://doi.org/10.1073/pnas.0804529105
Argueso, Juan Lucas, James Westmoreland, Piotr A. Mieczkowski, Malgorzata Gawel, Thomas D. Petes, and Michael A. Resnick. “Double-strand breaks associated with repetitive DNA can reshape the genome.Proc Natl Acad Sci U S A 105, no. 33 (August 19, 2008): 11845–50. https://doi.org/10.1073/pnas.0804529105.
Argueso JL, Westmoreland J, Mieczkowski PA, Gawel M, Petes TD, Resnick MA. Double-strand breaks associated with repetitive DNA can reshape the genome. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11845–50.
Argueso, Juan Lucas, et al. “Double-strand breaks associated with repetitive DNA can reshape the genome.Proc Natl Acad Sci U S A, vol. 105, no. 33, Aug. 2008, pp. 11845–50. Pubmed, doi:10.1073/pnas.0804529105.
Argueso JL, Westmoreland J, Mieczkowski PA, Gawel M, Petes TD, Resnick MA. Double-strand breaks associated with repetitive DNA can reshape the genome. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11845–11850.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

August 19, 2008

Volume

105

Issue

33

Start / End Page

11845 / 11850

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Oligonucleotide Array Sequence Analysis
  • Models, Genetic
  • Genome, Fungal
  • DNA Repair
  • DNA Breaks, Double-Stranded
  • DNA
  • Chromosomes, Fungal