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Reduced levels of DNA polymerase delta induce chromosome fragile site instability in yeast.

Publication ,  Journal Article
Lemoine, FJ; Degtyareva, NP; Kokoska, RJ; Petes, TD
Published in: Mol Cell Biol
September 2008

Specific regions of genomes (fragile sites) are hot spots for the chromosome rearrangements that are associated with many types of cancer cells. Understanding the molecular mechanisms regulating the stability of chromosome fragile sites, therefore, has important implications in cancer biology. We previously identified two chromosome fragile sites in Saccharomyces cerevisiae that were induced in response to the reduced expression of Pol1p, the catalytic subunit of DNA polymerase alpha. In the study presented here, we show that reduced levels of Pol3p, the catalytic subunit of DNA polymerase delta, induce instability at these same sites and lead to the generation of a variety of chromosomal aberrations. These findings demonstrate that a change in the stoichiometry of replicative DNA polymerases results in recombinogenic DNA lesions, presumably double-strand DNA breaks.

Duke Scholars

Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

September 2008

Volume

28

Issue

17

Start / End Page

5359 / 5368

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Reproduction
  • Recombination, Genetic
  • Phenotype
  • Nucleic Acid Hybridization
  • Mutation
  • Molecular Sequence Data
  • Mitosis
  • Haploidy
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lemoine, F. J., Degtyareva, N. P., Kokoska, R. J., & Petes, T. D. (2008). Reduced levels of DNA polymerase delta induce chromosome fragile site instability in yeast. Mol Cell Biol, 28(17), 5359–5368. https://doi.org/10.1128/MCB.02084-07
Lemoine, Francene J., Natasha P. Degtyareva, Robert J. Kokoska, and Thomas D. Petes. “Reduced levels of DNA polymerase delta induce chromosome fragile site instability in yeast.Mol Cell Biol 28, no. 17 (September 2008): 5359–68. https://doi.org/10.1128/MCB.02084-07.
Lemoine FJ, Degtyareva NP, Kokoska RJ, Petes TD. Reduced levels of DNA polymerase delta induce chromosome fragile site instability in yeast. Mol Cell Biol. 2008 Sep;28(17):5359–68.
Lemoine, Francene J., et al. “Reduced levels of DNA polymerase delta induce chromosome fragile site instability in yeast.Mol Cell Biol, vol. 28, no. 17, Sept. 2008, pp. 5359–68. Pubmed, doi:10.1128/MCB.02084-07.
Lemoine FJ, Degtyareva NP, Kokoska RJ, Petes TD. Reduced levels of DNA polymerase delta induce chromosome fragile site instability in yeast. Mol Cell Biol. 2008 Sep;28(17):5359–5368.

Published In

Mol Cell Biol

DOI

EISSN

1098-5549

Publication Date

September 2008

Volume

28

Issue

17

Start / End Page

5359 / 5368

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Reproduction
  • Recombination, Genetic
  • Phenotype
  • Nucleic Acid Hybridization
  • Mutation
  • Molecular Sequence Data
  • Mitosis
  • Haploidy