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Analysis of the proteins involved in the in vivo repair of base-base mismatches and four-base loops formed during meiotic recombination in the yeast Saccharomyces cerevisiae.

Publication ,  Journal Article
Stone, JE; Petes, TD
Published in: Genetics
July 2006

DNA mismatches are generated when heteroduplexes formed during recombination involve DNA strands that are not completely complementary. We used tetrad analysis in Saccharomyces cerevisiae to examine the meiotic repair of a base-base mismatch and a four-base loop in a wild-type strain and in strains with mutations in genes implicated in DNA mismatch repair. Efficient repair of the base-base mismatch required Msh2p, Msh6p, Mlh1p, and Pms1p, but not Msh3p, Msh4p, Msh5p, Mlh2p, Mlh3p, Exo1p, Rad1p, Rad27p, or the DNA proofreading exonuclease of DNA polymerase delta. Efficient repair of the four-base loop required Msh2p, Msh3p, Mlh1p, and Pms1p, but not Msh4p, Msh5p, Msh6p, Mlh2p, Mlh3p, Exo1p, Rad1p, Rad27p, or the proofreading exonuclease of DNA polymerase delta. We find evidence that a novel Mlh1p-independent complex competes with an Mlhp-dependent complex for the repair of a four-base loop; repair of the four-base loop was affected by loss of the Mlh3p, and the repair defect of the mlh1 and pms1 strains was significantly smaller than that observed in the msh2 strain. We also found that the frequency and position of local double-strand DNA breaks affect the ratio of mismatch repair events that lead to gene conversion vs. restoration of Mendelian segregation.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

July 2006

Volume

173

Issue

3

Start / End Page

1223 / 1239

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Pyrophosphatases
  • Mutation
  • MutS Homolog 2 Protein
  • Meiosis
  • Developmental Biology
  • DNA, Fungal
  • DNA Repair Enzymes
 

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

July 2006

Volume

173

Issue

3

Start / End Page

1223 / 1239

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Pyrophosphatases
  • Mutation
  • MutS Homolog 2 Protein
  • Meiosis
  • Developmental Biology
  • DNA, Fungal
  • DNA Repair Enzymes