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Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites.

Publication ,  Journal Article
Lemoine, FJ; Degtyareva, NP; Lobachev, K; Petes, TD
Published in: Cell
March 11, 2005

In the yeast Saccharomyces cerevisiae, reduced levels of the replicative alpha DNA polymerase result in greatly elevated frequencies of chromosome translocations and chromosome loss. We selected translocations in a small region of chromosome III and found that they involve homologous recombination events between yeast retrotransposons (Ty elements) on chromosome III and retrotransposons located on other chromosomes. One of the two preferred sites of these translocations on chromosome III involve two Ty elements arrayed head-to-head; disruption of this site substantially reduces the rate of translocations. We demonstrate that this pair of Ty elements constitutes a preferred site for double-strand DNA breaks when DNA replication is compromised, analogous to the fragile sites observed in mammalian chromosomes.

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

March 11, 2005

Volume

120

Issue

5

Start / End Page

587 / 598

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Saccharomyces cerevisiae
  • Retroelements
  • Recombination, Genetic
  • Oligonucleotide Array Sequence Analysis
  • Genome, Fungal
  • Gene Expression Regulation, Fungal
  • Developmental Biology
  • DNA Replication
  • DNA Repair
 

Citation

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Lemoine, F. J., Degtyareva, N. P., Lobachev, K., & Petes, T. D. (2005). Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites. Cell, 120(5), 587–598. https://doi.org/10.1016/j.cell.2004.12.039
Lemoine, Francene J., Natasha P. Degtyareva, Kirill Lobachev, and Thomas D. Petes. “Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites.Cell 120, no. 5 (March 11, 2005): 587–98. https://doi.org/10.1016/j.cell.2004.12.039.
Lemoine FJ, Degtyareva NP, Lobachev K, Petes TD. Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites. Cell. 2005 Mar 11;120(5):587–98.
Lemoine, Francene J., et al. “Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites.Cell, vol. 120, no. 5, Mar. 2005, pp. 587–98. Pubmed, doi:10.1016/j.cell.2004.12.039.
Lemoine FJ, Degtyareva NP, Lobachev K, Petes TD. Chromosomal translocations in yeast induced by low levels of DNA polymerase a model for chromosome fragile sites. Cell. 2005 Mar 11;120(5):587–598.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

March 11, 2005

Volume

120

Issue

5

Start / End Page

587 / 598

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Saccharomyces cerevisiae
  • Retroelements
  • Recombination, Genetic
  • Oligonucleotide Array Sequence Analysis
  • Genome, Fungal
  • Gene Expression Regulation, Fungal
  • Developmental Biology
  • DNA Replication
  • DNA Repair