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The compact chromatin structure of a Ty repeated sequence suppresses recombination hotspot activity in Saccharomyces cerevisiae.

Publication ,  Journal Article
Ben-Aroya, S; Mieczkowski, PA; Petes, TD; Kupiec, M
Published in: Mol Cell
July 23, 2004

Recombination between repeated DNA sequences can have drastic consequences on the integrity of the genome. Repeated sequences are abundant in most eukaryotes, yet the mechanism that prevents recombination between them is currently unknown. Ty elements, the main family of dispersed repeats in Saccharomyces cerevisiae, exhibit low levels of exchange. Other regions in the genome have relatively high rates of meiotic recombination (hotspots). We show that a Ty element adjacent to the HIS4 recombination hotspot substantially reduces its activity, eliminating local DSB formation. We demonstrate that the Ty has a closed (nuclease-insensitive) chromatin configuration that is also imposed on the flanking DNA sequences. The compact chromatin structure is determined by sequences at the N terminus of the Ty. Increased binding of the Rap1 protein to the hotspot restores both open chromatin conformation and DSB formation. The chromatin configuration of Ty elements precludes initiation of recombination, thus preventing potentially lethal exchanges between repeated sequences.

Duke Scholars

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

July 23, 2004

Volume

15

Issue

2

Start / End Page

221 / 231

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Telomere-Binding Proteins
  • Suppression, Genetic
  • Shelterin Complex
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • Recombination, Genetic
  • Pyrophosphatases
 

Citation

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Ben-Aroya, S., Mieczkowski, P. A., Petes, T. D., & Kupiec, M. (2004). The compact chromatin structure of a Ty repeated sequence suppresses recombination hotspot activity in Saccharomyces cerevisiae. Mol Cell, 15(2), 221–231. https://doi.org/10.1016/j.molcel.2004.06.002
Ben-Aroya, Shay, Piotr A. Mieczkowski, Thomas D. Petes, and Martin Kupiec. “The compact chromatin structure of a Ty repeated sequence suppresses recombination hotspot activity in Saccharomyces cerevisiae.Mol Cell 15, no. 2 (July 23, 2004): 221–31. https://doi.org/10.1016/j.molcel.2004.06.002.
Ben-Aroya S, Mieczkowski PA, Petes TD, Kupiec M. The compact chromatin structure of a Ty repeated sequence suppresses recombination hotspot activity in Saccharomyces cerevisiae. Mol Cell. 2004 Jul 23;15(2):221–31.
Ben-Aroya, Shay, et al. “The compact chromatin structure of a Ty repeated sequence suppresses recombination hotspot activity in Saccharomyces cerevisiae.Mol Cell, vol. 15, no. 2, July 2004, pp. 221–31. Pubmed, doi:10.1016/j.molcel.2004.06.002.
Ben-Aroya S, Mieczkowski PA, Petes TD, Kupiec M. The compact chromatin structure of a Ty repeated sequence suppresses recombination hotspot activity in Saccharomyces cerevisiae. Mol Cell. 2004 Jul 23;15(2):221–231.

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

July 23, 2004

Volume

15

Issue

2

Start / End Page

221 / 231

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Transcription Factors
  • Telomere-Binding Proteins
  • Suppression, Genetic
  • Shelterin Complex
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repetitive Sequences, Nucleic Acid
  • Recombination, Genetic
  • Pyrophosphatases