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Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae.

Publication ,  Journal Article
Mallory, JC; Bashkirov, VI; Trujillo, KM; Solinger, JA; Dominska, M; Sung, P; Heyer, WD; Petes, TD
Published in: DNA Repair (Amst)
September 18, 2003

In eukaryotes, mutations in a number of genes that affect DNA damage checkpoints or DNA replication also affect telomere length [Curr. Opin. Cell Biol. 13 (2001) 281]. Saccharomyces cerevisae strains with mutations in the TEL1 gene (encoding an ATM-like protein kinase) have very short telomeres, as do strains with mutations in XRS2, RAD50, or MRE11 (encoding members of a trimeric complex). Xrs2p and Mre11p are phosphorylated in a Tel1p-dependent manner in response to DNA damage [Genes Dev. 15 (2001) 2238; Mol. Cell 7 (2001) 1255]. We found that Xrs2p, but not Mre11p or Rad50p, is efficiently phosphorylated in vitro by immunopreciptated Tel1p. Strains with mutations eliminating all SQ and TQ motifs in Xrs2p (preferred targets of the ATM kinase family) had wild-type length telomeres and wild-type sensitivity to DNA damaging agents. We also showed that Rfa2p (a subunit of RPA) and the Dun1p checkpoint kinase, which are required for DNA damage repair and which are phosphorylated in response to DNA damage in vivo, are in vitro substrates of the Tel1p and Mec1p kinases. In addition, Dun1p substrates with no SQ or TQ motifs are phosphorylated by Mec1p in vitro very inefficiently, but retain most of their ability to be phosphorylated by Tel1p. We demonstrated that null alleles of DUN1 and certain mutant alleles of RFA2 result in short telomeres. As observed with Xrs2p, however, strains with mutations of DUN1 or RFA2 that eliminate SQ motifs have no effect on telomere length or DNA damage sensitivity.

Duke Scholars

Published In

DNA Repair (Amst)

DOI

ISSN

1568-7864

Publication Date

September 18, 2003

Volume

2

Issue

9

Start / End Page

1041 / 1064

Location

Netherlands

Related Subject Headings

  • Transformation, Genetic
  • Transcription Factors
  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Replication Protein A
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Precipitin Tests
  • Plasmids
 

Citation

APA
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Mallory, J. C., Bashkirov, V. I., Trujillo, K. M., Solinger, J. A., Dominska, M., Sung, P., … Petes, T. D. (2003). Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. DNA Repair (Amst), 2(9), 1041–1064. https://doi.org/10.1016/s1568-7864(03)00115-0
Mallory, Julia C., Vladimir I. Bashkirov, Kelly M. Trujillo, Jachen A. Solinger, Margaret Dominska, Patrick Sung, Wolf Dietrich Heyer, and Thomas D. Petes. “Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae.DNA Repair (Amst) 2, no. 9 (September 18, 2003): 1041–64. https://doi.org/10.1016/s1568-7864(03)00115-0.
Mallory JC, Bashkirov VI, Trujillo KM, Solinger JA, Dominska M, Sung P, et al. Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. DNA Repair (Amst). 2003 Sep 18;2(9):1041–64.
Mallory, Julia C., et al. “Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae.DNA Repair (Amst), vol. 2, no. 9, Sept. 2003, pp. 1041–64. Pubmed, doi:10.1016/s1568-7864(03)00115-0.
Mallory JC, Bashkirov VI, Trujillo KM, Solinger JA, Dominska M, Sung P, Heyer WD, Petes TD. Amino acid changes in Xrs2p, Dun1p, and Rfa2p that remove the preferred targets of the ATM family of protein kinases do not affect DNA repair or telomere length in Saccharomyces cerevisiae. DNA Repair (Amst). 2003 Sep 18;2(9):1041–1064.
Journal cover image

Published In

DNA Repair (Amst)

DOI

ISSN

1568-7864

Publication Date

September 18, 2003

Volume

2

Issue

9

Start / End Page

1041 / 1064

Location

Netherlands

Related Subject Headings

  • Transformation, Genetic
  • Transcription Factors
  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Replication Protein A
  • Protein Serine-Threonine Kinases
  • Protein Kinases
  • Precipitin Tests
  • Plasmids