Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast.
Publication
, Journal Article
Sia, EA; Dominska, M; Stefanovic, L; Petes, TD
Published in: Mol Cell Biol
December 2001
The stability of simple repetitive DNA sequences (microsatellites) is a sensitive indicator of the ability of a cell to repair DNA mismatches. In a genetic screen for yeast mutants with elevated microsatellite instability, we identified strains containing point mutations in the yeast mismatch repair genes, MSH2, MSH3, MLH1, and PMS1. Some of these mutations conferred phenotypes significantly different from those of null mutations in these genes. One semidominant MSH2 mutation was identified. Finally we showed that strains heterozygous for null mutations of mismatch repair genes in diploid strains in yeast confer subtle defects in the repair of small DNA loops.
Duke Scholars
Published In
Mol Cell Biol
DOI
ISSN
0270-7306
Publication Date
December 2001
Volume
21
Issue
23
Start / End Page
8157 / 8167
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Point Mutation
- Phenotype
- Mutagenesis
- MutS Homolog 3 Protein
- MutS Homolog 2 Protein
- MutL Proteins
- MutL Protein Homolog 1
- Microsatellite Repeats
Citation
APA
Chicago
ICMJE
MLA
NLM
Sia, E. A., Dominska, M., Stefanovic, L., & Petes, T. D. (2001). Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast. Mol Cell Biol, 21(23), 8157–8167. https://doi.org/10.1128/MCB.21.23.8157-8167.2001
Sia, E. A., M. Dominska, L. Stefanovic, and T. D. Petes. “Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast.” Mol Cell Biol 21, no. 23 (December 2001): 8157–67. https://doi.org/10.1128/MCB.21.23.8157-8167.2001.
Sia EA, Dominska M, Stefanovic L, Petes TD. Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast. Mol Cell Biol. 2001 Dec;21(23):8157–67.
Sia, E. A., et al. “Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast.” Mol Cell Biol, vol. 21, no. 23, Dec. 2001, pp. 8157–67. Pubmed, doi:10.1128/MCB.21.23.8157-8167.2001.
Sia EA, Dominska M, Stefanovic L, Petes TD. Isolation and characterization of point mutations in mismatch repair genes that destabilize microsatellites in yeast. Mol Cell Biol. 2001 Dec;21(23):8157–8167.
Published In
Mol Cell Biol
DOI
ISSN
0270-7306
Publication Date
December 2001
Volume
21
Issue
23
Start / End Page
8157 / 8167
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae Proteins
- Saccharomyces cerevisiae
- Point Mutation
- Phenotype
- Mutagenesis
- MutS Homolog 3 Protein
- MutS Homolog 2 Protein
- MutL Proteins
- MutL Protein Homolog 1
- Microsatellite Repeats