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Protein kinase activity of Tel1p and Mec1p, two Saccharomyces cerevisiae proteins related to the human ATM protein kinase.

Publication ,  Journal Article
Mallory, JC; Petes, TD
Published in: Proc Natl Acad Sci U S A
December 5, 2000

The Saccharomyces cerevisiae proteins Tel1p and Mec1p are involved in telomere length regulation and cellular responses to DNA damage. The closest relative of these proteins is the human Ataxia Telangiectasia Mutated (ATM) protein, a wortmannin-sensitive protein kinase that primarily phosphorylates serines in an SQ motif. We constructed yeast strains containing functional epitope-tagged versions of Tel1p and Mec1p. We showed that immunoprecipitated Tel1p and Mec1p were capable of in vitro phosphorylation of the mammalian protein PHAS-I (Phosphorylated Heat and Acid Stable protein). These activities are sensitive to wortmannin. Tel1p phosphorylates serine in an SQ motif in PHAS-I. Mutations in the kinase domains of Tel1p and Mec1p result in loss of in vitro kinase activity and the in vivo phenotypes associated with the null tel1 and mec1 mutations.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 5, 2000

Volume

97

Issue

25

Start / End Page

13749 / 13754

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Phosphoproteins
  • Intracellular Signaling Peptides and Proteins
  • Humans
  • Fungal Proteins
  • DNA-Binding Proteins
 

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Mallory, J. C., & Petes, T. D. (2000). Protein kinase activity of Tel1p and Mec1p, two Saccharomyces cerevisiae proteins related to the human ATM protein kinase. Proc Natl Acad Sci U S A, 97(25), 13749–13754. https://doi.org/10.1073/pnas.250475697
Mallory, J. C., and T. D. Petes. “Protein kinase activity of Tel1p and Mec1p, two Saccharomyces cerevisiae proteins related to the human ATM protein kinase.Proc Natl Acad Sci U S A 97, no. 25 (December 5, 2000): 13749–54. https://doi.org/10.1073/pnas.250475697.
Mallory, J. C., and T. D. Petes. “Protein kinase activity of Tel1p and Mec1p, two Saccharomyces cerevisiae proteins related to the human ATM protein kinase.Proc Natl Acad Sci U S A, vol. 97, no. 25, Dec. 2000, pp. 13749–54. Pubmed, doi:10.1073/pnas.250475697.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 5, 2000

Volume

97

Issue

25

Start / End Page

13749 / 13754

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Phosphoproteins
  • Intracellular Signaling Peptides and Proteins
  • Humans
  • Fungal Proteins
  • DNA-Binding Proteins