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Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes.

Publication ,  Journal Article
Kirkpatrick, DT; Wang, YH; Dominska, M; Griffith, JD; Petes, TD
Published in: Mol Cell Biol
November 1999

Tandem repeats of the pentanucleotide 5'-CCGNN (where N indicates any base) were previously shown to exclude nucleosomes in vitro (Y. -H. Wang and J. D. Griffith, Proc. Natl. Acad. Sci. USA 93:8863-8867, 1996). To determine the in vivo effects of these sequences, we replaced the upstream regulatory sequences of the HIS4 gene of Saccharomyces cerevisiae with either 12 or 48 tandem copies of CCGNN. Both tracts activated HIS4 transcription. We found that (CCGNN)(12) tracts elevated meiotic recombination (hot spot activity), whereas the (CCGNN)(48) tract repressed recombination (cold spot activity). In addition, a "pure" tract of (CCGAT)(12) activated both transcription and meiotic recombination. We suggest that the cold spot activity of the (CCGNN)(48) tract is related to the phenomenon of the suppressive interactions of adjacent hot spots previously described in yeast (Q.-Q. Fan, F. Xu, and T. D. Petes, Mol. Cell. Biol. 15:1679-1688, 1995; Q.-Q. Fan, F. Xu, M. A. White, and T. D. Petes, Genetics 145:661-670, 1997; T.-C. Wu and M. Lichten, Genetics 140:55-66, 1995; L. Xu and N. Kleckner, EMBO J. 16:5115-5128, 1995).

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

November 1999

Volume

19

Issue

11

Start / End Page

7661 / 7671

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription Factors
  • Tandem Repeat Sequences
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Regulatory Sequences, Nucleic Acid
  • Recombination, Genetic
  • Pyrophosphatases
  • Nucleosomes
  • Mutagenesis, Insertional
 

Citation

APA
Chicago
ICMJE
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Kirkpatrick, D. T., Wang, Y. H., Dominska, M., Griffith, J. D., & Petes, T. D. (1999). Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes. Mol Cell Biol, 19(11), 7661–7671. https://doi.org/10.1128/MCB.19.11.7661
Kirkpatrick, D. T., Y. H. Wang, M. Dominska, J. D. Griffith, and T. D. Petes. “Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes.Mol Cell Biol 19, no. 11 (November 1999): 7661–71. https://doi.org/10.1128/MCB.19.11.7661.
Kirkpatrick DT, Wang YH, Dominska M, Griffith JD, Petes TD. Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes. Mol Cell Biol. 1999 Nov;19(11):7661–71.
Kirkpatrick, D. T., et al. “Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes.Mol Cell Biol, vol. 19, no. 11, Nov. 1999, pp. 7661–71. Pubmed, doi:10.1128/MCB.19.11.7661.
Kirkpatrick DT, Wang YH, Dominska M, Griffith JD, Petes TD. Control of meiotic recombination and gene expression in yeast by a simple repetitive DNA sequence that excludes nucleosomes. Mol Cell Biol. 1999 Nov;19(11):7661–7671.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

November 1999

Volume

19

Issue

11

Start / End Page

7661 / 7671

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription Factors
  • Tandem Repeat Sequences
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Regulatory Sequences, Nucleic Acid
  • Recombination, Genetic
  • Pyrophosphatases
  • Nucleosomes
  • Mutagenesis, Insertional