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Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain.

Publication ,  Journal Article
Kirkpatrick, DT; Fan, Q; Petes, TD
Published in: Genetics
May 1999

The DNA sequences located upstream of the yeast HIS4 represent a very strong meiotic recombination hotspot. Although the activity of this hotspot requires the transcription activator Rap1p, the level of HIS4 transcription is not directly related to the level of recombination. We find that the recombination-stimulating activity of Rap1p requires the transcription activation domain of the protein. We show that a hybrid protein with the Gal4p DNA-binding domain and the Rap1p activation domain can stimulate recombination in a strain in which Gal4p-binding sites are inserted upstream of HIS4. In addition, we find recombination hotspot activity associated with the Gal4p DNA-binding sites that is independent of known transcription factors. We suggest that yeast cells have two types of recombination hotspots, alpha (transcription factor dependent) and beta (transcription factor independent).

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

May 1999

Volume

152

Issue

1

Start / End Page

101 / 115

Location

United States

Related Subject Headings

  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Recombinant Fusion Proteins
  • Pyrophosphatases
  • Plasmids
  • Models, Genetic
  • Models, Biological
  • Minisatellite Repeats
 

Citation

APA
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ICMJE
MLA
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Kirkpatrick, D. T., Fan, Q., & Petes, T. D. (1999). Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain. Genetics, 152(1), 101–115. https://doi.org/10.1093/genetics/152.1.101
Kirkpatrick, D. T., Q. Fan, and T. D. Petes. “Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain.Genetics 152, no. 1 (May 1999): 101–15. https://doi.org/10.1093/genetics/152.1.101.
Kirkpatrick, D. T., et al. “Maximal stimulation of meiotic recombination by a yeast transcription factor requires the transcription activation domain and a DNA-binding domain.Genetics, vol. 152, no. 1, May 1999, pp. 101–15. Pubmed, doi:10.1093/genetics/152.1.101.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

May 1999

Volume

152

Issue

1

Start / End Page

101 / 115

Location

United States

Related Subject Headings

  • Transcription Factors
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Recombinant Fusion Proteins
  • Pyrophosphatases
  • Plasmids
  • Models, Genetic
  • Models, Biological
  • Minisatellite Repeats