Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Fine-structure mapping of meiosis-specific double-strand DNA breaks at a recombination hotspot associated with an insertion of telomeric sequences upstream of the HIS4 locus in yeast.

Publication ,  Journal Article
Xu, F; Petes, TD
Published in: Genetics
July 1996

Meiotic recombination in Saccharomyces cerevisiae is initiated by double-strand DNA breaks (DSBs). Using two approaches, we mapped the position of DSBs associated with a recombination hotspot created by insertion of telomeric sequences into the region upstream of HIS4. We found that the breaks have no obvious sequence specificity and localize to a region of approximately 50 bp adjacent to the telomeric insertion. By mapping the breaks and by studies of the exonuclease III sensitivity of the broken ends, we conclude that most of the broken DNA molecules have blunt ends with 3'-hydroxyl groups.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

July 1996

Volume

143

Issue

3

Start / End Page

1115 / 1125

Location

United States

Related Subject Headings

  • Transcription Factors
  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Pyrophosphatases
  • Polymerase Chain Reaction
  • Mutagenesis, Insertional
  • Molecular Sequence Data
  • Meiosis
 

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

July 1996

Volume

143

Issue

3

Start / End Page

1115 / 1125

Location

United States

Related Subject Headings

  • Transcription Factors
  • Telomere
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Pyrophosphatases
  • Polymerase Chain Reaction
  • Mutagenesis, Insertional
  • Molecular Sequence Data
  • Meiosis