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Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences.

Publication ,  Journal Article
Schiestl, RH; Dominska, M; Petes, TD
Published in: Mol Cell Biol
May 1993

When the yeast Saccharomyces cerevisiae was transformed with DNA that shares no homology to the genome, three classes of transformants were obtained. In the most common class, the DNA was inserted as the result of a reaction that appears to require base pairing between the target sequence and the terminal few base pairs of the transforming DNA fragment. In the second class, no such homology was detected, and the transforming DNA was integrated next to a CTT or GTT in the target; it is likely that these integration events were mediated by topoisomerase I. The final class involved the in vivo ligation of transforming DNA with nucleus-localized linear fragments of mitochondrial DNA.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1993

Volume

13

Issue

5

Start / End Page

2697 / 2705

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Sequence Homology, Nucleic Acid
  • Saccharomyces cerevisiae
  • Plasmids
  • Mutagenesis, Insertional
  • Molecular Sequence Data
  • Genes, Fungal
  • Gene Deletion
  • Developmental Biology
  • DNA, Ribosomal
 

Citation

APA
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ICMJE
MLA
NLM
Schiestl, R. H., M. Dominska, and T. D. Petes. “Transformation of Saccharomyces cerevisiae with nonhomologous DNA: illegitimate integration of transforming DNA into yeast chromosomes and in vivo ligation of transforming DNA to mitochondrial DNA sequences.Mol Cell Biol 13, no. 5 (May 1993): 2697–2705. https://doi.org/10.1128/mcb.13.5.2697-2705.1993.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

May 1993

Volume

13

Issue

5

Start / End Page

2697 / 2705

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Sequence Homology, Nucleic Acid
  • Saccharomyces cerevisiae
  • Plasmids
  • Mutagenesis, Insertional
  • Molecular Sequence Data
  • Genes, Fungal
  • Gene Deletion
  • Developmental Biology
  • DNA, Ribosomal